The development and immune recognition of natural killer (NK) cell are regulated critically by major histocompatibility complex (MHC) class I molecules. However, it remains unclear whether the function of NK cells is regulated by MHC class II molecules. To test this, we monitored the development, phenotype and function of NK cells by using MHC class II deficient (H2-/-) mice. The numbers and development of NK cells keep unaltered in H2-/- mice, compared with those in wide type (H2+/+) mice. A part of Ly49 family receptors on NK cells are down-regulated both in mRNA and protein expression in absence of MHC class II molecules. Furthermore, NK cells obtained from H2-/- mice exhibit more expression of CD69 and IFN-γ after cross-linking with NK1.1. Also, the cytotoxicity against tumor cell lines of NK cells from H2-/- mice was increased significantly. Taken together, our study indicates that the absence of MHC class II molecules promotes the activation and function of NK cells in mice.Certain natural products, derived from medicinal plants, exhibit anti-inflammatory properties, but the mechanism of action of many remains unclear. Borrelia burgdorferi spirochetes are responsible for causing Lyme arthritis through activation of the Toll-like receptor (TLR) signaling pathway. In this study, we investigated the mechanisms by which Isoforskolin (ISOF) and Cucurbitacin IIa (CuIIa), compounds derived from Chinese herbs, can exert anti-inflammatory effects by modulating single immunoglobulin interleukin-1 receptor-related receptor (SIGIRR; also known as Toll/interleukin-1 receptor 8, TIR8) and thereby inhibiting B. burgdorferi basic membrane protein A (BmpA)-induced TLR signaling in human macrophages, specifically the THP-1 human monocytic cell line. After THP-1 cells were exposed in vitro to i) recombinant (r)BmpA, ii) rBmpA and ISOF or iii) rBmpA and CuIIa, Cytotoxicity assay (Cell Counting Kit-8, CCK-8) are used to measure the effects of ISOF and CuIIa on cell viability. Meanwhile, real-time polymerase chain reaction and Western blotting were used to quantify SIGIRR mRNA and protein levels, respectively, at 6, 12, 24 and 48 h time points post-stimulation. In addition, proinflammatory cytokine tumor necrosis factor-α (TNF-α) was determined by ELISA analysis. Our study showed that rBmpA stimulation of THP-1 cells resulted in a drop in SIGIRR levels in THP-1 cells. ML355 More importantly, SIGIRR levels increased significantly in rBmpA-stimulated THP-1 cells following ISOF or CuIIa administration, and the results of ELISA analysis suggested that ISOF or CuIIa reduced the secretion of the proinflammatory cytokine TNF-α. In conclusion, These results reveal new possibilities for the treatment of Lyme arthritis.At present, the occurrence and pollution of microplastics have caused widespread concern, but there are still few studies on inland lake and its affiliated rivers. In this study, we monitored the existence and characteristics of microplastics in Dongting Lake and its affiliated rivers. Our sampling site covers almost the entire lake district. A total of 15 surface water samples and 15 sediment samples were collected. The abundance of microplastics achieved 0.62-4.31 items/m2 in plankton net samples, with 21-52 items/100 g dw in sediments. The microplastics were dominated by fiber, 0.9-0.333 mm of surface water and ˂0.1 mm of sediments in size. The color of transparent occupied the majority in all microplastics samples. There was no significant difference in the average concentration of microplastics between the four river mouths and the whole lake and the rivers may have important potential contribution to the microplastics pollution in the lake.Due to their widespread use, organophosphate esters (OPEs) are commonly detected in various environmental matrices and have been identified as emerging contaminants. In this review article, the occurrence and analytical techniques of OPEs in the biotic environment have been compiled and reviewed. Data from studies published the last decade all over the world covering a variety of species in trophic chain have been synthesized and evaluated. OPEs are among the most frequent detected flame retardants and high concentrations are detected in biota to date. Knowledge gaps and recommendations for future research are discussed emphasizing on further monitoring, advanced analytical methodologies, and risk assessment studies to completely understand the science of OPEs in biota. This study aimed to describe the psychosocial reactions to relocation to a nursing home from the perspective of older adults. This study applied a qualitative interview design. Narratives from 23 Chinese nursing home residents in a life review program in Fuzhou, China were recorded, transcribed into sentences, and analyzed using qualitative content analysis. The following psychosocial reactions to relocation to a nursing home emerged fear, struggle, compromise, acceptance, and contribution. The fear resulted from negative labels attached to nursing homes, fear of disconnection to society, fear of difficulties in establishing new relationships, and fear of being abandoned by their families. The behaviors of 'struggle' were complaining about family members, thinking of returning home, praying for change, and taking action to leave. The keys to compromise were a choice between maintaining harmony in family relations and the companionship of relatives, a choice between professional care and family care, and a choice between the cost and effectiveness of family care and nursing home care. The process of acceptance included accepting life yet with worries, affirming the benefits of living in a nursing home, and embracing nursing home life. Contribution was mainly reflected in two aspects, a sense of ownership and bring into full play their self-worth. This study generates new insights into knowledge on the psychosocial reactions to relocation to a nursing home. It provides both family members and nursing home staff with direction on how to ensure a smoother relocation process.This study generates new insights into knowledge on the psychosocial reactions to relocation to a nursing home. It provides both family members and nursing home staff with direction on how to ensure a smoother relocation process.