In this study, the common pattern observed was that PSB were the most abundant in all types of soils compared to other traits. Conversely, most of the isolates, which are salt-tolerant, copper-tolerant, and ampicillin-resistant, showed phosphate solubilization activity. The sequencing of the partial 16S-rRNA gene revealed that PSB belonged to Bacillus genera. The online version contains supplementary material available at 10.1007/s13205-021-02904-7.The online version contains supplementary material available at 10.1007/s13205-021-02904-7.Drought stress is the main growth-limiting factor in pigeon pea production. Plant growth-promoting bacteria (PGPB) induce abiotic stress tolerance in several plants. However, the physiological and molecular changes with PGPB priming are not well understood in pigeon pea. The present study explored the potential of Firmibacteria (Bacillus azotoformans MTCC2953, Bacillus aryabhattai KSBN2K7, and Paenibacillus stellifer M3T4B6) to induce stress tolerance in pigeon pea under pot culture condition. Different physiological and biochemical parameters, including osmolytes, stress enzymes, and antioxidants, were evaluated under two stress conditions (50% and 25% field capacity) and an unstressed condition in pigeon pea. Under moisture stress conditions significant differences were observed in physiological and biochemical parameters between firmibacteria inoculated and control plants.The quantitative real-time polymerase chain reaction was performed to study the bacterial inoculation mediated expression of proline and drought-responsive genes in enhancing the drought tolerance in pigeon pea. Results showed that the inoculation of Bacillus aryabhattai upregulated the expression of drought-responsive genes (C. cajan_29830 and C. cajan_33874) and downregulated the expression of the proline gene by inducing the drought stress tolerance in inoculated plants compared with the uninoculated control plants. Therefore, Bacillus aryabhattai may be recommended for inducing drought stress tolerance and increasing the growth of pigeon pea under moisture stress conditions after field evaluation.The biosynthesis of cysteine is crucial and critically regulated by two enzymes. i.e., serine acetyl transferase (SAT) and O-acetyl serine (thiol) lyase (OAS-TL). A descriptive account on the activity and regulatory mechanism of the enzyme is available in bacteria and plants. But no such studies yet performed in cyanobacteria, to understand the evolutionary aspect of cysteine biosynthesis and its regulation. Therefore, in our study a detailed bioinformatic analysis has been performed to understand all the possible features of cyanobacterial SATs and OAS-TLs. The analysis of SAT and OAS-TL sequences from cyanobacteria depicted that the large genome and morphological complexities favoured acquisition of these genes. Besides, conserved function of these enzymes was presumed by their sequence similarity. Further, the phylogenetic tree consisted of distinct clusters for unicellular, filamentous, and heterocytous strains. Nevertheless, the specificity pocket, SVKDR for OAS-TL having K as catalytic residue was also identified. selleck chemicals llc Additionally, in silico protein modelling of SAT (SrpG) and OAS-TL (SrpH) of Synechococcus elongatus PCC 7942 was performed to gain insight into the structural attributes of the proteins. Finally, here we showed the possibility of hetero-oligomeric bi-enzyme cysteine synthase complex formation upon interaction of SAT and OAS-TL through protein-protein docking analysis thus provides a way to understand the regulation of cysteine biosynthesis in cyanobacteria. The online version contains supplementary material available at 10.1007/s13205-021-02899-1.The online version contains supplementary material available at 10.1007/s13205-021-02899-1.Sporisorium scitamineum is a teleomorphic, biotrophic fungus causing the globally prevalent sugarcane smut disease in sugarcane. The severity of the disease depends on two major factors, viz. degree of resistance in the host genotype and virulence level of the pathogen. Hence, in this study, temporal transcriptomic expression of potential pathogenicity-associated genes of two distinctly virulent S. scitamineum isolates, viz. SsV89101 (low virulent) and Ss97009 (high virulent) were analyzed during interaction with a smut susceptible sugarcane cv. Co 97009 at six different time intervals. The pathogenicity-associated genes profiled in this study comprises 14 plant cell wall degrading enzymes (PCWDEs) and ten candidates secreted effector protein-coding (CSEPs) genes. Absolute quantification of pathogen biomass and comparative expression profiling analyses of these pathogenicity-associated genes during host-pathogen interaction indicated that there was a significant variation between low and high virulent isolate1-02893-7.The online version contains supplementary material available at 10.1007/s13205-021-02893-7.The present study was undertaken to study the function of miRNA-199-3p in the regulation of human lung cancer growth and metastasis. The results showed significant (P  less then  0.05) downregulation of miRNA-199-3p in lung cancer tissues and cell lines. Overexpression of miR-197 caused considerable inhibition of the viability and colony formation of the lung cancer cells. The inhibition of proliferation was found to be due to the arrest of the SK-LU-1 lung cancer cells. At the G2/M phase of the cell cycle. In silico analysis and subsequent the dual-luciferase assays showed that miR-199-3p targets Sp1 at molecular. The expression of Sp1 was significantly (P  less then  0.05) upregulated in lung cancer cells and tissues. Nonetheless, miR-199-3p overexpression could cause post-transcriptional suppression of Sp1. Silencing of Sp1suppress the proliferation of SK-LU-1 lung cancer cells. However, overexpression Sp1 transcription factor prevents the tumor-suppressive effects of miR-199-3p on lung cancer cells. Additionally, miR-199-3p was found to suppresses the migration, invasion and epithelial-to-mesenchymal transition of human lung cancer cells. Summing up, miRNA-199-3p/SP1 axis controls the growth and metastasis of SK-LU-1 lung cancer cells.