Genome mining of the bacterial strains Pseudomonas sp. SH-C52 and Pseudomonas fluorescens DSM 11579 showed that both strains contained a highly similar gene cluster encoding an octamodular nonribosomal peptide synthetase (NRPS) system which was not associated with a known secondary metabolite. Insertional mutagenesis of an NRPS component followed by comparative profiling led to the discovery of the corresponding novel linear octalipopeptide thanafactin A, which was subsequently isolated and its structure determined by two-dimensional NMR and further spectroscopic and chromatographic methods. In bioassays, thanafactin A exhibited weak protease inhibitory activity and was found to modulate swarming motility in a strain-specific manner.Drug-induced acute liver injury (DIALI) is increasingly recognized as a significant cause of acute liver injury (ALI), which is characterized by a rapid loss of hepatocyte function in patients without pre-existing liver diseases. Evaluation of corresponding biomarkers, including alanine transaminase and aspartate amino transferase, is available as a diagnostic tool for hepatotoxicity. However, these blood tests have certain limitations (1) they are generally not available for early estimation; (2) it is difficult to visualize and identify hepatotoxicity unambiguously in real-time; and (3) the biomarkers are not unique and are usually influenced by a variety of diseases, leading to potential false results. It is of grave importance and burgeoning demand to develop an early diagnostic approach for such diseases, but the ideal toolkit remains an unresolved challenge.As an alternative, molecular optical probes (fluorescence, chemiluminescence, bioluminescence, etc.) display a lot of advantages, such as high sensierlying liver diseases, and to improve the efficiency of the diagnosis and treatment of these diseases in clinical settings.A new compound [Gd(hfac)3(MeOTEMPO)(MeOH)] (MeOTEMPO = 4-methoxy-2,2,6,6-tetramethylpiperidin-1-oxyl) was prepared. From the X-ray crystal structure analysis, the Gd-O-N angle is 170.9(3)°. The magnetic study clarified the Gd3+-radical interaction with 2J/kB = -26.6(3) K (in the H = -2JS1·S2 convention), which corresponds to one of the strongest antiferromagnetic couplings in the Gd-nitroxide systems. Wider Gd-O-N angles seem to favor stronger antiferromagnetic couplings.The molecular structure of Sc3N@C2v(7854)-C70 was determined by single-crystal X-ray diffraction. Variable-temperature X-ray diffraction analysis unraveled the details of the phase transition caused by the temperature-driven jumplike rotation of the fullerene cage between two orientations. Whereas in the lower-temperature P21/c phase the fullerene predominantly occupies one orientation, two orientations become equally occupied in the higher-temperature C2/m phase. This work provides a rare example of the well-defined order-disorder transition in metallofullerene crystals and thus gives important insight into the problem of disorder impeding metallofullerene crystallography.Here we describe the formation of an unexpected and unique family of hollow six-stranded helicates. The formation of these structures depends on the coordinative flexibility of silver and the 2-formyl-1,8-napthyridine subcomponent. Crystal structures show that these assemblies are held together by Ag4I, Ag4Br, or Ag6(SO4)2 clusters, where the templating anion plays an integral structure-defining role. Prior to the addition of the anionic template, no six-stranded helicate was observed to form, with the system instead consisting of a dynamic mixture of triple helicate and tetrahedron. Six-stranded helicate formation was highly sensitive to the structure of the ligand, with minor modifications inhibiting its formation. This work provides an unusual example of mutual stabilization between metal clusters and a self-assembled metal-organic cage. The selective preparation of this anisotropic host demonstrates new modes of guiding selective self-assembly using silver(I), whose many stable coordination geometries render design difficult.In crystalline solids, molecules generally have limited mobility due to their densely packed environment. However, structural information at the molecular level may be used to design amphidynamic crystals with rotating elements linked to rigid, lattice-forming parts, which may lead to molecular rotary motions and changes in conformation that determine the physical properties of the solid-state materials. Here, we report a novel design of emissive crystalline molecular rotors with a central pyrazine rotator connected by implanted transition metals (Cu or Au) to a readily accessible enclosure formed by two N-heterocyclic carbenes (NHC) in discrete binuclear complexes. The activation energies for the rotation could be tuned by changing the implanted metal. Exchanging Cu to Au resulted in an ∼4.0 kcal/mol reduction in the rotational energy barrier as a result of lower steric demand by elongation of the axle with the noble metal, and a stronger electronic stabilization in the rotational transition state by enhancement of the d-π* interactions between the metal centers and the pyrazine rotator. The Cu(I) rotor complex showed a greater electronic delocalization than the Au(I) rotor complex, causing a red-shifted solid-state emission. selleck chemical Molecular rotation-induced emission quenching was observed in both crystals. The enclosing NHC rotors are easy to prepare, and their rotational motion should be less dependent on packing structures, which are often crucial for many previously documented amphidynamic molecular crystals. The platform from the encapsulating NHC cationic metal complexes and the metal-centered rotation-axis provide a promising scaffold for a novel design of crystalline molecular rotors, including manipulation of rotary dynamics and solid-state emission.In terms of documented references, multifunctional MOFs with high catalytic performance could be constructed from the combination of metal cations and polycarboxyl-pyridine ligands, which could efficiently endow crystallized porous frameworks with the coexisting Lewis acid-base properties. Thus, by employing a ligand-directed synthetic strategy, the exquisite combination of wave-like inorganic chains of [Tm(CO2)3(OH2)] n and mononuclear units of [Tm(CO2)4(OH2)2] with the aid of the specially designed ligand of 2,6-bis(2,4-dicarboxylphenyl)-4-(4-carboxylphenyl)pyridine (H5BDCP) generates one highly robust microporous framework of (Me2NH2)[Tm3(BDCP)2)(H2O)3]·4DMF·H2O n (simplified as NUC-25), which contains near-rectangular nanochannels and large solvent-residing voids. Furthermore, the activated state of NUC-25 with the removal of associated water molecules is a rarely reported bifunctional heterogeneous catalyst due to the coexistence of Lewis acid-base sites including 6-coordinated Tm3+ ions, uncoordinated carboxyl oxygen atoms, and Npyridine atoms.