This study investigated the effect of a recoverable sulphuric acid and sodium hydroxide-modified pinewood (MOP) as a bulking agent during sewage sludge and sawdust composting (MOPC), with a control experiment using unpretreated pinewood (UNP; UNPC) as the bulking agent. Results show that addition of MOP effectively promoted the degradation of organic matter during composting. The maximum temperature increased by 1.50 °C and the high temperature period (T > 50 °C) of composting was extended 4 days longer than the control experiment. Furthermore, MOP addition reduced the loss of nitrogen by 9.40%. High-throughput sequencing analysis showed that the bacterial communities in the UNPC and MOPC treatments were significantly different. Pseudoxanthomonas was the dominant bacteria during the thermophilic and cooling phases of the MOPC treatment. In addition, the recycling efficiency of the UNP and MOP was 99.18% and 99.37%, respectively. Aerobic denitrification is attracting increasing attention since its advantage of complete nitrogen removal in a single aerobic reactor with simplified configurations. This study investigated the nitrate kinetic affinity (half-saturation index, Km) by an isolated aerobic denitrifier named P. balearica strain RAD-17. It turned out that strain RAD-17 had a high Km of 162.5 mg-N/L and maximum nitrate reduction rate of 21.7 mg-N/(L•h), enabling it to treat high-strength nitrogen wastewater with high efficiency. Further analysis illustrated that Km was the critical value for the change of growth yield rate along initial nitrate concentrations. Nitrogen balance results elucidated an opposite nitrogen flux to cell synthesis and nitrogen loss during aerobic denitrification. Moreover, the expression of functional genes provided proofs for these phenotypic results at transcriptional level. Consequently, Km could be an indicator for nitrate flux division directing to respiration and assimilation in aerobic denitrifiers, shedding light on its regulation for wastewater treatment. Anodic metabolic rate showed regulatory influence on the desalination performance of microbial desalination cell (MDC) under open (OC) and closed circuit (CC) operations. In this study, three MDCs were tested for desalination with three different organic substrate loads 1500 ± 55 mg/L in MDC-A; 3500 ± 10 g/L in MDC-B; 4500 ± 12 g/L in MDC-C. Higher desalination and substrate removal rates were observed in CC than OC. Average desalination was MDC-C (51.4%-CC) > MDC-B (47.3%-CC) > MDC-A (45.3%-CC) and COD removal efficiencies were MDC-C (68.4%-CC) > MDC-B (64.4%-CC) > MDC-A (51.9%-CC). Increase in organic load resulted in higher desalination efficiency which was due to higher electrochemical and ionic gradient apart from anodic metabolic activity. The voltage and current density were observed to be maximum in MDC-C (685 mV; 2.16 mA/m2) followed by MDC-B (598 mV; 1.98 mA/m2) and MDC-A (501 mV; 1.76 mA/m2). This study demonstrated that the MDCs performance can be regulated by varying organic load and circuitry modes. The physiological properties, including biochemical composition and cell wall thickness, of microalgal species have a remarkable effect on the pretreatment of biomass and its further conversion to biofuels. In the present study, multiple biofuels (bioethanol, higher alcohols (C3-C5), and biodiesel) were produced using energy-efficient microwave pretreatment, successive carbohydrate/protein fermentation, and lipid transesterification from three microalgal strains (Pseudochlorella sp., Chlamydomonas mexicana, and Chlamydomonas pitschmannii). The microwave pretreatment method required the lowest specific energy (5 MJ/kg) compared to ultrasound pretreatment. The proposed integrated approach achieved high conversion efficiency (46%) and maximum biomass utilization (93%) of C. mexicana with improved yields of bioethanol (0.46 g-ethanol/g-carbohydrates), higher alcohols (0.44 g-higher alcohols/g-proteins), and biodiesel (0.74 g-biodiesel/g-lipids). This study suggests that the application of an appropriate pretreatment method for microalgal strains having different physiological properties is essential for improving the extraction efficiency and conversion of biomass to biofuels with less waste production. Extracellular electron transfer (EET) is a significant pathway to transport electrons between bacteria and electrode in microbial electrosynthesis systems (MESs). To enhance EET in the MES, a high-conductivity polymer, polypyrrole (PPy), was coated on the surface of mixed culture acetogens in situ and the PPy-coated bacteria were inoculated on the cathode of MES. The charge transfer resistance of PPy-coated biocathode was 33%-70% of that with PPy-uncoated. Acetate production rate and Faradic efficiency in PPy-coated biocathodes increased by 3 to 6 times. read more After 960 h operation, Acetobacterium, Desulfovibrio, and Acinetobacter dominate the community on the coated and uncoated biocathode. Quinone loop and NADH dehydrogenase to ubiquinone were involved in electron transfer pathway of biocathode and stimulated by PPy coating. Low-level expression of C-type cytochromes on biocathode indicated its less important role in inward EET. The study provided useful information for applications of high-conductivity chemicals in microbial electrosynthesis. A combined bead milling and enzymatic hydrolysis process was developed for fractionation of the major valuable biomass components, i.e., proteins, carbohydrates, and lipids from the microalgae Chlorella vulgaris. The cells were treated by bead milling followed by hydrolysis with different hydrolytic enzymes, including lipase, phospholipase, protease, and cellulase. Without enzymatic hydrolysis, the recovery yield of lipids, carbohydrates, and proteins for bead milled biomass was 75%, 31%, and 40%, respectively, while by applying enzymatic treatments these results were improved significantly. The maximum recovery yield for all components was obtained after enzymatic hydrolysis of bead milled biomass by lipase at 37 °C and pH 7.4 for 24 h, yielding 88% lipids in the solid phase while 74% carbohydrate and 68% protein were separated in the liquid phase. The recovery yield of components after enzymatic hydrolysis of biomass without bead milling was 44% lower than that of the milled biomass.