In this paper, a novel acidic polysaccharide (CPS-1) was successively prepared from Gynostemma pentaphyllum using hot water isolation method to explore its antitumor and antioxidant activities. Structural characteristics of CPS-1 were evaluated by SEM, HPGPC, HPAEC-PAD, FT-IR, and NMR. The results indicated CPS-1 was mainly composed of Ara, Gal, Glc, Xyl, Man, GalA and GlcA in a molar ratio of 1.232.140.670.20.290.160.04 with molecular weight of 3297 kDa. Combining with the results of FT-IR and NMR, it was inferred that CPS-1 was mainly possessed the five main linkages including α-D-Ara, α-D-Gal, α-D-Man, α-D-Xyl and β-D-Glc. Furthermore, MTT results exhibited that the IC50 value of CPS-1 for inhibitive effect on SPC-A-1 and MGC-803 cells for 24 h were 284.36 and 365.27 μg/mL, respectively. Microscopic observations showed that the cells exhibited significant apoptotic characteristics, such as cell shrinkage, the decreased of cell adherence and the appearance of apoptotic bodies. It was shown that CPS-1 had significant anti-tumor activity. In addition, the ability of CPS-1 to scavenge superoxide radical, ABTS and DPPH radicals was also enhanced with the increased of concentration. Therefore, it was revealed that CPS-1 might be used as a natural anticancer and antioxidant component.The severe effects of pharmaceutical and personal care products (PPCPs) could not be ignored and they must be eliminated prior to their release to the environment. In this study, cellulosic sisal fibre was modified simultaneously by polypyrrole-polyaniline nanoparticles and it was employed as a cost-effective, non-toxic nano bio-composite for the elimination of ibuprofen. It was characterized by SEM, EDAX, FTIR, and XRD. Parameters were tested in the form of the one-factor-at-a-time method. These parameters were contact time, pH, initial ibuprofen concentration, adsorbent dosage, agitation speed, and temperature and the optimized conditions obtained were 60 min, 5, 30 mg/L, 150 mg, 200 rpm, and 313 K, respectively and ibuprofen removal efficiency reached 88%. Furthermore, Kinetics data were fitted on the Pseudo-second model (R2 0.9991), indicating a chemisorption process. The Isothermal study demonstrated that Sips assumptions had the greatest R2 value in the examined temperatures (R2 0.9985 at 298 K). Energy site distribution revealed that at the higher temperature more binding sites were activated on the modified cellulosic Sisal, resulted in greater adsorption capacity, with the highest capacity of 19.45 mg/g (based on the Langmuir model) at 313 K. Modified cellulosic Sisal can be concluded to be a cost-effective, prominent, and efficient adsorbent for ibuprofen removal.The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A). In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans. To enhance the biological activity, we used to demonstrated that chitosan and spicules as a drug carrier. The Callophycin A loading was confirmed by spectral variation of FT-IR and morphological variation by SEM. Moreover, around 65% and 38% of Cal A was successfully loaded in chitosan and spicules respectively. Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi. After 6 days of treatment Cal@Chi produces a significant reduction in the fungal burden of vaginal lavage. The histo-morphological alterations also evidenced that the protective role of Cal@Chi in VVC model. The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites. Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.Polydatin (PD) is a bio-active ingredient with known anti-tumor effects. However, its specific protein targets yet have not been systematically screened, and the molecular anti-tumor mechanism is still unclear. Here, proteomic-chip was efficiently used to screen potential targets of PD. First, we investigated through animal experiment and proteomics studies, and found that polydatin play an important role in tumor cells. Then, the red-green fluorescent of polydatin was compared comprehensively to screen its targets on chip, followed by bioinformatics analysis. Glutathione synthetase (GSS) was selected as candidate research target. After a series of molecular biological experiments GSS was confirmed a target protein for PD in vitro. Moreover, we also found that PD can significantly inhibit the activity of GSS in vitro and glutathione synthesis in live cells. Our findings reveal that PD could be a selective small-molecule GSS enzyme activity inhibitor and GSS could be a potential therapeutic target in cancer.Epilobium is a medicinal plant; its extracts are widely used traditional medicine due to their broad range of pharmacological and therapeutic properties. Its most prominent feature is its therapeutic effects on prostatic diseases. The aim of this study is preparation of controlled release system of Epilobium parviflorum, and determination of its potential of anticancer applications. For this purpose, Epilobium parviflorum extract (EPE) loaded chitosan nanoparticles were prepared with ionic gelation method to increase the bioavailability of the extract. The nanoparticles were investigated in terms of size, zeta potential, polydispersity index, encapsulation efficiency, loading capacity and release profile. Besides, scanning electron microscopy (SEM) was used to observe the morphology of the nanoparticles. CA3 Moreover, Ames/Salmonella test was used to determine the mutagenicity of EPE, and it was shown that it had no mutagenic effect. It was found that EPE loaded chitosan nanoparticles were with 64.47 nm in average size, 0.168 PdI and 15.2 mV zeta potential. Encapsulation efficiency and loading capacity were found as 92.46% and 8%, respectively. Finally, DNA binding assay and in silico molecular docking studies were performed between EPE and DNA in order to contribute to design of plant based controlled release system for use in cancer therapy.