spottaiwan22
spottaiwan22
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The authors suggest that the improved quality of the NIL may be due to the genomic segment carried along with the Lr24/Sr24 genes. The online version contains supplementary material available at 10.1007/s13205-021-02679-x.The online version contains supplementary material available at 10.1007/s13205-021-02679-x.Konjac glucomannan oligosaccharide has attracted much attention due to its broad biological activities. Specific glucomannan degrading enzymes are effective tools for the production of oligosaccharides from konjac glucomannan. However, there are still few reports of commercial enzymes that can specifically degrade konjac glucomannan. The gene ppgluB encoding a glucomannanase consisting of 553 amino acids (61.5 kDa) from Paenibacillus polymyxa 3-3 was cloned and heterologous expressed in Escherichia coli BL21 (DE3). The recombinant PpGluB showed high specificity for the degradation of konjac glucomannan. Moreover, the hydrolytic products of PpGluB degrade konjac glucomannan were a series of oligosaccharides with degrees of polymerisation of 2-12. Furthermore, the biochemical properties indicated that PpGluB is the optimal active at 45 to 55 °C and pH 5.0-6.0, and shows highly pH stability over a very broad pH range. The present characteristics indicated that PpGluB is a potential tool to be used to produce oligosaccharides from konjac glucomannan.The purpose of this study was to identify biomarkers and construct a diagnostic prediction model for multiple sclerosis (MS). Microarray datasets in the Gene Expression Omnibus (GEO) were downloaded. Weighted gene coexpression analysis (WGCNA) was used to search for hub modules and biomarkers related to MS. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to roughly define their biological functions and pathways. Least absolute shrinkage and selection operator (LASSO) regression and multivariate logistic regression analysis were used to identify the diagnostic biomarkers and construct a nomogram. The calibration curve and receiver operating characteristic (ROC) curve were used to judge the diagnostic predictive ability. In addition, cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) algorithm was used to calculate the proportion of 22 kinds of immune cells. GSE41850 was used as the training set, and GSE17048 was used as the test set. WGCNA revealed one hub module containing 165 hub genes. Most of their biological functions and pathways are related to cell metabolism and immune cell activation. The diagnostic nomogram contained ARPC5, ROD1, UBQLN2, ZNF281, ABCA1 and FAS. The ROC curve and the calibration curve of the training set and test set confirmed that the nomogram had great prediction ability. In addition, monocytes and M0 macrophages were significantly different between MS patients and healthy people. The expression of ARPC5, ZNF281 and ABCA1 is correlated with M0 macrophages. The nomogram provides new insights and contributes to the accurate diagnosis of MS. The online version contains supplementary material available at 10.1007/s13205-021-02675-1.The online version contains supplementary material available at 10.1007/s13205-021-02675-1.We report the case of a 52-year-old hyperglycemic woman with type 2 diabetes and severe coronavirus disease 2019 (COVID-19)-associated pneumonia, possibly involving the subcutaneous insulin resistance (SIR) syndrome. After admission for pneumonia, her average daily blood glucose (BG) levels remained at 300-400 mg/dL, although the required dosage of subcutaneous insulin markedly increased (~ 150 units/day; ~ 2.63 units/kg/day). Furthermore, the patient had generalized edema along with hypoalbuminemia, developed extensive abdominal purpuras, and had increased plasma D-dimer levels during treatment, suggestive of coagulation abnormalities. Therefore, intravenous infusion of regular insulin was initiated. Celastrol mw The BG level subsequently decreased to  The online version contains supplementary material available at 10.1007/s13340-021-00500-x.The online version contains supplementary material available at 10.1007/s13340-021-00500-x.Results from the NASA Van Allen Probes mission indicate extensive observations of mirror/drift-mirror (M/D-M hereafter) unstable plasma regions in the night-side inner magnetosphere. Said plasmas lie on the threshold between the kinetic and frozen-in plasma regimes and have favorable conditions for the formation of M/D-M modes and subsequent ultralow frequency (ULF) wave signatures in the surrounding plasma. We present the results of a climatological analysis of plasma-γ (anisotropy measure) and total plasma-β (ratio of particle to magnetic field pressure) in regard to the satisfaction of instability conditions on said M/D-M modes under bi-Maxwellian distribution assumption, and ascertain the most likely region for such plasmas to occur. Our results indicate a strong preference for the premidnight sector of the night-side magnetosphere, with events ranging in time scales from half a minute (roughly 200 km in scale size) to several hours (multiple Earth radii). The statistical distribution of these plasma regions explicitly identifies the source region of "storm time Pc5 ULF waves" and suggests an alternative mechanism for their generation in the night-side inner magnetosphere.The early Earth was marked by at least one global magma ocean. Melt buoyancy played a major role for its evolution. Here we model the composition of the magma ocean using a six-component pyrolite melt, to which we add volatiles in the form of carbon as molecular CO or CO2 and hydrogen as molecular H2O or through substitution for magnesium. We compute the density relations from first-principles molecular dynamics simulations. We find that the addition of volatiles renders all the melts more buoyant compared to the reference volatile-free pyrolite. The effect is pressure dependent, largest at the surface, decreasing to about 20 GPa, and remaining roughly constant to 135 GPa. The increased buoyancy would have enhanced convection and turbulence, and thus promoted the chemical exchanges of the magma ocean with the early atmosphere. We determine the partial molar volume of both H2O and CO2 throughout the magma ocean conditions. We find a pronounced dependence with temperature at low pressures, whereas at megabar pressures the partial molar volumes are independent of temperature.

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