94 before intervention to 4.40 ± 1.87 following the workshop (P value less then .001). As for the intended practices, the educational intervention improved the participants' intent to exercise, eat healthy foods, and do annual screening. Conclusions Participants' knowledge, attitudes, and intended practices improved following a trained pharmacy led educational intervention. These encouraging results highlight the need for continuous national educational interventions about diabetes provided by trained health care professionals such as pharmacists. Future studies are needed to evaluate the impact of improved knowledge and perceptions on actual long-term practice.Host-parasite relationships are likely to change over the coming decades in response to climate change and increased anthropogenic stressors. Understanding the genetic architecture of parasite resistance will aid prediction of species' responses to intensified parasite challenge. The gapeworm "Syngamus trachea" is prevalent in natural bird populations and causes symptomatic infections ranging from mild to severe. The parasite may affect ecological processes by curtailing bird populations and is important due to its propensity to spread to commercially farmed birds. Our large-scale data set on an insular house sparrow metapopulation in northern Norway includes information on gapeworm prevalence and infection intensity, allowing assessment of the genetics of parasite resistance in a natural system. To determine whether parasite resistance has a heritable genetic component, we performed variance component analyses using animal models. Resistance to gapeworm had substantial additive genetic and dominance variance, and genome-wide association studies to identify single nucleotide polymorphisms associated with gapeworm resistance yielded multiple loci linked to immune function. Together with genome partitioning results, this indicates that resistance to gapeworm is under polygenic control in the house sparrow, and probably in other bird species. Hence, our results provide the foundation needed to study any eco-evolutionary processes related to gapeworm infection, and show that it is necessary to use methods suitable for polygenic and nonadditive genetic effects on the phenotype.The death of a loved one may precipitate symptoms of prolonged grief disorder (PGD), post-traumatic stress disorder (PTSD) and depression. Brooding about the causes and consequences of one's negative affect (NA), also termed depressive rumination, has been linked to distress after loss. The role of dysregulation of positive affect (PA) has received less attention. We examined (1) the factor structure of depressive rumination and PA dysregulation and (2) to what extent these factors were related to PGD, PTSD and depression symptom levels. Self-report data were included from 235 Dutch bereaved people who completed measures tapping symptoms of PGD, PTSD and depression. Depressive rumination and PA regulation strategies were assessed with the Ruminative Response Scale (RRS) Brooding Scale and the Response to Positive Affect (RPA) Questionnaire (including three subscales emotion-focused and self-focused rumination and dampening), respectively. Confirmatory factor analyses and structural equation modelling were used for data analyses. The four-factor model (i.e., depressive rumination and the three RPA subscales) showed the best fit. An increase in depressive rumination was related to higher distress levels (across all outcomes in univariate and multivariate analyses). An increase in emotion-focused rumination about PA was associated with less depression. More dampening of PA was related to higher PTSD levels. Findings suggest that, alongside the regulation of NA, the regulation of PA plays a role in how people respond to the death of a loved one. This points to the need for more research on NA and PA regulation in grief.A high amount of blood and not the mere presence of blood in equine semen impacts fertility. Epigenetic inhibitor The aim of this study was to develop an approach to rescue the fertility of stallions with high hemospermia levels. Semen from 15 stallions was divided into four experimental groups (1) Control-pure raw semen, (2) WB50-50% (v/v) whole blood added into semen, (3) E1-WB50 extended in a 11 (v/v) ratio with milk-based extender, and (4) E2-WB50 extended in a 21 ratio with milk-based extender. Sperm kinetics, plasma membrane integrity (PMI), lipid peroxidation (PER), and intracellular superoxide (O2 ) production were immediately evaluated. Four cycles of 20 mares were randomly assigned to the experimental groups. Mares were bred with an insemination dose of 1 billion total sperm and pregnancy was diagnosed 14 days after ovulation. Sperm kinetics could not be evaluated in the WB50 samples. Total motility was lower (P 0.05) for CT and E1 samples. The control (90%) and E2 (90%) groups had superior (P less then 0.05) fertility than the others (WB50-0% and E1-25%). It was concluded that sperm motility and fertility of semen with a large amount of blood can be rescued by dilution with a 21 extendersemen ratio using a milk-based extender.The use of hydrodynamic cavitation (HC) as a wastewater treatment and anaerobic digestion pretreatment is a well-known process. However, most of the experiments have been done at laboratory scale and using a low concentration of total solids in the sludge treated. In this study, the waste-activated sludge has been mixed with pig slurry with the aim of treating two wastes rich in nutrients and organic matter. The HC has been studied not only at laboratory scale but also at industrial scale (up to 500 L), using a novel rotating device consisting of a rotor with multiple teeth that rotate inside a grooved stator. The effectiveness of the process has been calculated using the disintegration degree (DD) and analyzing the volatile fatty acids, while the energy efficiency has been determined with specific energy of the sludge solubilization (SESCOD ) and the specific energy. Results show that both the SESCOD and the specific energy decrease when the cavitation process is scaled from laboratory scale to industrial scale.