Graphene oxide (GO) will enter the soil environment in increasing amounts. learn more The effects of GO on the dissipation of benzo[a]pyrene (B[a]P) from contaminated soil and their phytoremediation system have been explored in this study. B[a]P is a ubiquitous soil pollutant used as a representative indicator of polycyclic aromatic hydrocarbons. A pot experiment was performed to investigate the effects of GO or/and vegetation (Tagetes patula) on B[a]P dissipation and the associated bacterial communities in soil. The bacterial communities in soil were investigated by Illumina sequencing analysis. The presence of vegetation significantly enhanced the dissipation of B[a]P from soil. The addition of GO (100 mg/kg) significantly decreased the B[a]P dissipation. When vegetation and GO coexisted, the inhibition effects of GO on B[a]P dissipation were alleviated by vegetation. Compared with the control treatment, the presence of GO or vegetation had no significant effects on the richness and diversity of bacterial communities in B[a]P-contaminated soil. Compared with the presence of only vegetation, the richness and diversity all significantly decreased when vegetation and GO coexisted. And, vegetation had a greater influence on the bacterial community composition than GO. Vegetation alleviated the inhibition effects of GO on B[a]P dissipation and had a greater influence on the associated bacterial communities than GO. This work helps to understand the interactive effects of GO and vegetation on B[a]P dissipation and the associated bacterial communities in contaminated soil. The emergence and spread of antibiotic resistance are major threats to ecosystems and human health. Transoceanic channels (e.g., ship ballast water) can transfer harmful aquatic organisms across geographically isolated waters. However, the occurrence of antibiotic resistance genes (ARGs) in ship ballast water and their relationship with microbial communities and environmental factors remain unknown. In this study, ballast water from 28 vessels sailing to Shanghai and Jiangyin (China) were collected, and the ARGs in these water samples were investigated. Considerable levels of ARGs and integrase of the class-I integrons (intI1) were detected in all ballast water samples. sul1 and tetQ were the most and least abundant ARGs in ballast water samples, respectively. The ARGs were strongly correlated with those of the 16S rRNA and intI1 genes. Ballast water exchange can reduce the absolute abundance of some kinds of ARGs while increasing the relative abundance of several ARGs (e.g., mefA, mexF, strB, sul1, and tetQ). Moreover, the bacterial hosts of ARGs were generally different in the unexchanged ballast water (UEBW) and exchanged ballast water (EBW). In particular, Leisingera and unclassified_Erythrobacteraceae were the main ARGs-associated genera in the EBW, while Pseudohongiella, Cycloclasticus, OM43_clade, norank_f_Rhodospirillaceae, and norank_f_Rhodobacteraceae were the dominant ARGs hosts in the UEBW. Overall, ship ballast water is an effective moving carrier for the global transference of ARGs, and its sufficient management is required for mitigating ARGs propagation across oceans. The impact on H2S alleviation and methane yield enhancement after submitting the anaerobic digestion of chicken manure to a finite amount of air was investigated. The largest reduction in the H2S biogas content (58% lower) occurred when air intensity of 30 ml/g VSin was injected into the reactors. Consequently, a maximum methane yield (335 mL-g VSin-1), which was 77% higher than the control, was concurrently achieved. Slight sulfate accumulation ( less then 330 mg L-1) was observed inside the micro-aerated digesters with higher air intensities, suggesting a suppression of sulfide inhibition. Bacterial diversity/richness was enhanced in these digesters while the relative abundance of Methanocelleus increased by 36%. The most important contributing factor to enhancement was the synergistic effect resulting from increments in the hydrolysis rate and the suppression of sulfide inhibition. The results highlighted the potential of in situ H2S mitigation with the added benefit of methane yield enhancement. Nile perch wastewater was biodegraded using two Bacillus species to recover bioactive substances to enhance its reutilization value. The two Bacillus species successfully produced low-molecular-weight substances with a 47.8% degree of hydrolysis. The antioxidant activities of the Nile perch wastewater increased as the biodegradation proceeded, and the culture supernatant exhibited the highest DPPH (80.1%), ABTS (93.1%) and Fe2+ chelating (88.5%) antioxidant activities at 60 h. The antioxidant potential of the biodegraded Nile perch wastewater was found to be higher than those of other fish hydrolysates. Moreover, the biodegraded Nile perch wastewater exhibited effective antimicrobial activity against Vibrio vulnificus, exhibiting a minimal inhibitory concentration of 585 μg mL-1. Two-dimensional thin layer chromatography analysis revealed the specific amino acids responsible for the antioxidant activity, and molecular-weight cut-off ultrafiltration revealed that the less then 2-kDa fraction exhibited the highest antioxidant activity with the lowest IC50 values (0.43 and 0.22 mg mL-1 for DPPH and ABTS antioxidant activities, respectively). This is the first report of the reutilization of Nile perch wastewater as a natural antioxidant and antimicrobial ingredient for nutraceuticals. The role of illumination and cathode is important to improve the efficiency of photoelectro-Fenton (PEF) system. In this study, cathodes with black carbon-poly tetra fluoro ethylene (BC-PTFE) for increase the concentration of hydrogen peroxide in PEF. A new PEF system using EIEL and BC-PTFE air-diffusion cathode was established. The electrode performance was tested and the influence factors, degradation kinetics, intermediates, pathway and mechanism of the model compound methyl orange (MO) were studied. The capacities of concentration decays and total organic carbon (TOC) removals were compared between different electrochemical advanced oxidation processes. The experimental conditions were optimized for a current density of 20 mA cm-2 with 0.5 mM Fe2+ and 100 mg L-1 MO at 20 °C and pH 3.0 in an 8 L reservoir. The higher MO concentration was, the smaller pseudo-first-order kinetic constants of concentration decays and TOC removals were. Intermediate products were identified by gas chromatography-mass spectrometry and ion-exclusion high performance liquid chromatograph in EIEL-PEF.