Membrane identity, and the dynamic processes active in membrane regions, offer crucial signals for regulating transport, intercellular signaling, and communication across membranes. How membrane identities transform and dynamic processes at the membrane surface are regulated across diverse eukaryotes, especially in plants, remains an open question, particularly in regard to the roles played by protein complexes composed of both peripheral and integral membrane proteins. In eukaryotes, a class of conserved peripheral membrane proteins, the SEC14L-PITPs, is comprised of SEC14-like phosphatidylinositol transfer proteins. These proteins' common SEC14 domain is critical in defining membrane characteristics and regulating membrane trafficking activities. Its ability to sense, bind, move, and interchange lipophilic molecules, including phosphoinositides and numerous other lipid-soluble compounds, across membranes is essential. SEC14L-PITPs are found as SEC14-only proteins in every organism examined, or, in animals and streptophytes (including charales and land plants), as multi-domain proteins with a modular construction. The functional roles of SEC14L-PITPs are reviewed here, with a significant focus on the multi-domain SEC14L-PITPs in the SEC14-nodulin and SEC14-GOLD groups (PATELLINs, PATLs in plants). The diverse roles of SEC14L-PITPs in plants range from affecting membrane transport to affecting the overall well-being of the organism. The structural features of SEC14L-PITPs, their capability to bind both phospholipids and other lipophilic compounds, and their role in regulating complex cellular activities via protein-membrane interactions are the main subjects of our investigation.The perennial legume Sainfoin (Onobrychis spp.), traditionally used as a forage crop, is now attracting attention as a perennial pulse source for human consumption. Breeding and research projects heavily rely on sainfoin's dual use in improving its different lines for forage and pulses, which is the driving force behind the creation of intricate datasets about high-dimensional phenotypes within the post-omic era. For breeders selecting forage and those concentrating on edible seeds, accessing these rich datasets effectively hinges on the development of uniform ontologies and easily accessible ontology platforms. Crop Ontology, a platform initiated by the CGIAR in 2008, was designed to hold crop-specific trait ontologies, thus enabling standardized plant breeding databases. This study introduces the sainfoin crop ontology (CO). A comprehensive literature review was conducted to compile a detailed list of traits measured and reported for sainfoin. Given the capacity to measure identical attributes through various means and scales, researchers eventually established a set of 98 variables, each combining a plant trait, its measurement technique, and its corresponding scale, to thoroughly examine the diversity within sainfoin. For incorporation into the sainfoin CO, variables were formatted and standardized, in accordance with the accompanying guidelines. The 98 variables contained a collection of 82 traits; these traits were divided into four classes: 24 agronomic, 31 morphological, 19 of which were related to seed and forage quality, and 8 phenological. cftr signaling Furthermore, we've outlined a pathway for developing and submitting new sainfoin traits to the CO, in addition to the existing variables.The presence and concentration of plant pigments ultimately define the range of colors observed in the flowers. Angiosperms boast anthocyanins, the prevalent flavonoid pigments, which generate a wide spectrum of visible colors from red-magenta to deep blue-purple, originating from the distinct biosynthesis of cyanidin and delphinidin. Floral color, a significant aesthetic factor in the development of new commercial varieties within the floriculture industry, is nevertheless often constrained by the species' genetic limitations, thus defining a limited color spectrum. The absence of blue varieties in numerous decorative plants is attributable to the inactivity of vital biosynthetic enzymes required for the accumulation of delphinidin. In the realm of plant life, the poinsettia, Euphorbia pulcherrima Willd., stands out. Klotzsch, a Mexican-native Christmas ornamental plant, represents holiday spirit. The appeal of this flower stems from its bracts, altered leaves rich in reddish pigments chiefly cyanidin and, in a smaller proportion, pelargonidin. The foundation of this plant's commercial success is the creation of new cultivars; while the traditional red color remains popular, consumers also actively seek out poinsettias with innovative and uncommon color schemes. Earlier studies have emphasized the potential for modifying flower color through the metabolic engineering of the anthocyanin biosynthetic pathway and the application of plant tissue culture techniques in diverse ornamental plant types. Transgenic blossoms, including roses, carnations, and chrysanthemums, exhibit a striking, bluish coloration due to the high and exclusive concentration of delphinidin. This analysis assesses the viability of engineering the anthocyanin biosynthetic pathway in *E. pulcherrima* using the introduction of foreign delphinidin biosynthetic genes under the command of a pathway-specific promoter, and examines genome editing as an alternative technique for altering the hue of the bracts. Moreover, supplementary methods, such as the strategic choice of cultivars that optimize the intracellular conditions for delphinidin accumulation, alongside the introduction of genes encoding anthocyanin-modifying enzymes or transcription factors to encourage a bluer floral pigmentation, are also assessed.Salt stress, a major adverse environmental factor, acts as a significant limitation on plant growth. A noteworthy strategy for diminishing the negative impact of salt stress on plants involves the application of nitrogen. To investigate the N application mechanism's effect on alleviating salt stress in rapeseed seedlings, a pot experiment was carried out. Four N application levels (0, 0.01, 0.02, and 0.03 g N per kg soil), designated N0, N1, N2, and N3 respectively, were used in conjunction with non-salt (0 g NaCl per kg soil, S0) and salt stress (3 g NaCl per kg soil, S1) conditions. Under salt stress conditions, sodium content (23653%) and reactive oxygen species (ROS), particularly hydrogen peroxide (H2O2) (3026%), were elevated, resulting in cell membrane lipid peroxidation, indicated by a rise in malondialdehyde (MDA) (12232%) and a reduction in the photosynthetic rate (1559%), which ultimately led to inhibited plant growth, exemplified by shortened plant height, thinner root systems, reduced leaf area, and decreased dry weight. Application of N led to an increase in plant growth, and this improvement was more substantial in the presence of salt stress than in its absence. This indicates that rapeseed seedlings under salt stress are more reliant on nitrogen to fuel their growth. Seedlings under the influence of salt stress and nitrogen showed a reduction in reactive oxygen species levels, an uptick in photosynthetic activity, an increase in antioxidant levels including catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), and ascorbic acid (AsA), and elevated accumulation of osmotic substances such as soluble proteins, soluble sugars, and proline compared to seedlings without nitrogen. The best enhancement in response to salt stress by nitrogen application was observed at the N2 level; however, exceeding this level resulted in impaired nitrogen metabolism, thus decreasing the positive effects. The results of this study suggest that moderate nitrogen input can enhance photosynthesis, antioxidant processes, and osmoregulatory functions, thereby diminishing the negative effects of salt stress on young rapeseed plants.Plants that rely on ammonium-N often use a strategy that produces root exudates with biological nitrification inhibition (BNI) effectiveness.NH4+-NN-limited ecosystems harbor plant species that display remarkable tolerance. Studies on BNI have been predominantly based on plant species that are characteristic of acidic soils.Employing a multifaceted approach of field sampling and laboratory culture, we examined the capacity of BNI.Why a dominant grass species thrives in alkaline grasslands of eastern Asia was investigated.This subject is endowed with BNI capacity.The findings indicated thatThere is a strong capacity for BNI. With a concentration of one milligram per milliliter of solution,,Root exudates contributed to a reduction in nitrification levels within soils under their influence.The initial strategy showcased a 7244% reduction in the effect, notably exceeding the 6829% reduction observed in the subsequent method. Evaluating the soil's nitrification potential is important.The community's portion, representing 53%, of the whole group, was not fully comprehensive.Forty-one percent of the, or.The community's residents are connected through a web of social interactions. We went on to show that the availability ofNH4+-Ndriven byThe stipulations of BNI can be met. Along with this,NH4+-NNitrate-N uptake is influenced by pH regulation, which in turn enhances plant adaptation to alkaline stress conditions.NO3--NA list of sentences is the output of this JSON schema. Furthermore, we demonstrated the regulatory function ofNH4+-NIts nutritional function is outweighed by its alkaline environment. The data reveal groundbreaking knowledge concerning the manner in whichTo overcome high pH and nutrient deficiency challenges, the plant secretes BNIs, and for the first time, exposes differences in the functional roles of.NH4+-NandNO3--NThe growth and adaptation of a grass species are affected by alkaline environments.In the research, L. chinensis's capabilities related to BNI were found to be significant. In soils characterized by the presence of Puccinellia tenuiflora, 1 mg/mL of L. chinensis root exudates significantly reduced nitrification by 7244%, demonstrating a greater effect than DCD, which resulted in a 6829% inhibition.