Our results show that microgAMBI revealed a organically enriched environment along the gradient. However, the de novo biotic index outperformed microgAMBI by providing a higher discriminatory power in detecting changes in abiotic factors directly related to fish production, whilst allowing the identification of new ASVs bioindicators. The de novo strategy applied here represents a robust method to define new bioindicators in regions or habitats where no previous information about the response of bacteria to environmental stressors exists. Lymphoedema is an incurable and progressive disease that affects not only physical function but overall quality of life. Surgical treatment options for the management of lymphoedema are being increasingly performed. This study aims to review post-operative health-related quality of life (HRQOL) following surgical treatment of lymphoedema. A systematic search of the PubMed and Medline databases was performed from the date of their inception until September 2018 to evaluate HRQOL following different surgical options for the treatment of lymphoedema. One hundred and thirteen articles were identified. Twenty-one articles were included in the final review, comprising a total of 736 patients. HRQOL improvements appear to be sustained for at least 6-12 months post-operatively. In particular, major benefits were noted in the domains based around physical functioning. Patient satisfaction similarly mirrors HRQOL improvements, following an initial dip in the immediate post-operative period. All surgical treatment modalities for the management of lymphoedema confer significant HRQOL improvements across a diverse range of health domains, with this critical outcome of surgery an important pre-operative consideration. Recommendations for ongoing research are suggested.All surgical treatment modalities for the management of lymphoedema confer significant HRQOL improvements across a diverse range of health domains, with this critical outcome of surgery an important pre-operative consideration. Recommendations for ongoing research are suggested. Leisure cognitive activities are suggested to reduce the risk of dementia. Herein, we aimed to investigate the prospective association between engaging in different musical activities and the risk of dementia among Japanese older adults. Longitudinal data of 52 601 participants aged ≥65 years from the Japan Gerontological Evaluation Study were analyzed. Musical activities in the form of playing a musical instrument, practicing karaoke and choir or folk singing were assessed using a questionnaire, while dementia was diagnosed using the standardized dementia scale of the long-term care insurance system. The Cox regression was used to obtain the hazard ratios and 95% confidence intervals (CIs) for incident dementia according to engagement in musical activities. Compared with engaging in no musical activities at all, the hazard ratios engaging in one and more than one musical activity, after 5.8 years of a median follow-up period, were 0.94 (95% CI 0.82-1.07) and 0.59 (95% CI 0.32-1.10) in men versus 0.79 (95% CI 0.69-0.90) and 0.89 (95% CI 0.63-1.26) in women, respectively. Playing a musical instrument and practicing karaoke, compared with no musical activities at all, were associated with a faintly decreased risk of dementia in men and a significantly decreased risk of dementia in women; hazard ratios 0.70 (95% CI 0.45-1.02) and 0.90 (95% CI 0.79, 1.04) in men versus 0.75 (95% CI 0.58-0.98) and 0.77 (95% CI 0.68-0.89) in women, respectively. Engaging in musical activities, especially playing a musical instrument and practicing karaoke, was associated with a reduced risk of dementia among Japanese older women. Geriatr Gerontol Int 2021; 21 451-457.Engaging in musical activities, especially playing a musical instrument and practicing karaoke, was associated with a reduced risk of dementia among Japanese older women. Geriatr Gerontol Int 2021; 21 451-457. Surgery results in systemic inflammation, which can affect the central nervous system, leading to changes in mood, emotion, and behavior. Our previous study has shown that compared to midazolam, dexmedetomidine premedication effectively decreased children's postoperative anxiety. To investigate whether dexmedetomidine infusion before hernia repair alleviates postoperative systemic inflammation in children and whether postoperative anxiety may be associated with postoperative inflammation. This prospective double-blind randomized controlled trial was conducted in 120 children scheduled to undergo elective hernia repair. Before anesthesia induction, all children received an intravenous infusion consisted of dexmedetomidine (n=40; 0.5µg/g, group D), midazolam (n=40; 0.08mg/kg, group M), or normal saline (n=40; group C). learn more One-way ANOVA with least significant difference multiple comparison test was used for multigroup comparisons of postoperative plasma levels of inflammatory cytokines and m-YPAS scores. Speama levels of proinflammatory cytokines. A single preoperative intravenous dexmedetomidine dose in children undergoing same-day surgery reduces postoperative systemic inflammation.A single preoperative intravenous dexmedetomidine dose in children undergoing same-day surgery reduces postoperative systemic inflammation.The plant hypersensitive response (HR), a rapid cell death at the point of pathogenesis, is mediated by nucleotide-binding site, leucine-rich repeat (NLR) resistance proteins (R-proteins) that recognize the presence of specific pathogen-derived proteins. Rp1-D21 is an autoactive maize NLR R-protein that triggers HR spontaneously. We previously mapped loci associated with variation in the strength of HR induced by Rp1-D21. Here we identify the E3 ligase ZmMIEL1 as the causal gene at a chromosome 10 modifier locus. Transient ZmMIEL1 expression in Nicotiana benthamiana reduced HR induced by Rp1-D21, while suppression of ZmMIEL1 expression in maize carrying Rp1-D21 increased HR. ZmMIEL1 also suppressed HR induced by another autoactive NLR, the Arabidopsis R-protein RPM1D505V, in N. benthamiana. We demonstrated that ZmMIEL1 is a functional E3 ligase and that the effect of ZmMIEL1 was dependent on the proteasome but also that levels of Rp1-D21 and RPM1D505V were not reduced when coexpressed with ZmMIEL1 in the N. benthamiana system.