0001). No neurologic injuries occurred after 19 months of age. Among uninjured children followed prospectively from birth (Cohort I), measures of growth, nutritional sufficiency, motor development, and cognitive function were normal. Adherence to metabolic formula and l-carnitine supplementation in Cohort I declined to 12% and 32%, respectively, by age 7 years. Cessation of strict dietary therapy altered plasma amino acid and carnitine concentrations but resulted in no serious adverse outcomes. In conclusion, neonatal diagnosis of GA1 coupled to management with lysine-free, arginine-enriched metabolic formula and emergency IV infusions during the first two years of life is safe and effective, preventing more than 90% of striatal injuries while supporting normal growth and psychomotor development. The need for dietary interventions and emergency IV therapies beyond early childhood is uncertain.Microalgae accumulate lipid triacylglycerol (TAG), a promising feedstock for production of natural edible oils and biofuels. To make products derived from microalgal TAG economically viable, increasing TAG content and productivity are of high importance. To increase TAG content, two endogenous key enzymes of TAG biosynthesis plastidial lysophosphatidic acid acyltransferase (NeoLPAAT1) and endoplasmic reticulum-located diacylglycerol acyltransferase 2 (NeoDGAT2) were co-overexpressed in oleaginous microalga Neochloris oleoabundans. The neutral lipid content in NeoLPAAT1-NeoDGAT2 co-overexpressing transformant detected by Nile red staining increased 2-fold without compromising cell growth. The transcriptional levels of NeoLPAAT1 and NeoDGAT2 levels were 1.9-fold higher in the transformant than wild type. Considerably higher lipid accumulation was found in the transformant than wild type total lipid content (73.72 ± 4.17 % DCW) increased 1.6-fold, TAG content (50.63 ± 6.15 % DCW) increased 2.1-fold, total lipid productivity (16.84 ± 0.66 mg/L/day) increased 1.9-fold, and TAG productivity (11.68 ± 0.90 mg/L/day) increased 2.1-fold. Fatty acid composition was slightly altered in the transformant compared to wild type; saturated fatty acid C160 increased to 26% from 20%, whereas C180 was reduced to 7% from 14%. Long-term stability of NeoLPAAT1-NeoDGAT2 co-overexpression was observed in the transformant continuously maintained on solid medium in a period of 4 years. The results suggested that targeted engineering of genes in pathway located at different organelles should be possible in microalgal lipid metabolism.Unbalanced lipid peroxidation damages the human body, and is associated with the formation of tumors, infections, inflammations, autoimmune diseases, and cardiovascular and cerebrovascular diseases. However, food and drugs that contain anti-lipid peroxidation active substances, can help to protect against these negative health impacts. We observed lipid peroxidation inhibition in the metabolites of fermented Monascus anka, in media with Dendrobium nobile Lindl. The anti-lipid peroxidation ability of the extracts was strongest in ethyl acetate, so this was selected for further purifications. A crystal with strong antioxidant properties was obtained by column chromatography. Based on its spectroscopic analysis by Electron Bombardment Ion Source and Mass Spectrometry (EI-MS), 1H-Nuclear Magnetic Resonance (1H-NMR), and 13C-Nuclear Magnetic Resonance (13C-NMR), the isolated crystal was identified as ergosterol. The inhibition rates of the lipid peroxide due to the ergosterol were 57.42%, at 2μg/mL in vitro. Simultaneously, the survival rates of the damaged cells treated with 0.3mmol/L H2O2 were significantly improved with the ergosterol, up to 43.88% (200μg/mL) and 46.64% (400μg/mL), compared to 36.47% for the injured cells. The survival rate of the cells was 78.32% (400μg/mL), with ergosterol as a prevention. Cell injury can increase the level of intracellular ROS, but its levels in the damaged cells were reduced after the ergosterol treatments, and the reduction increased with the increasing concentrations. A 400μg/mL concentration resulted in the lowest fluorescence intensity; 33421.11 AU below the normal level. Ergosterol significantly reduced the ROS levels, to reduce the cell damage. Ergosterol from Monascus anka was thus found to have strong anti-lipid peroxidation and antioxidant capabilities, and the ability to protect and repair damaged cells. Rutin in vivo It may consequently serve as a potential natural antioxidant and will play an important role in human anti-lipid peroxide. To quantify the proportion of oral antibiotics requests without prescription in Community Pharmacy (CP) and to analyse it causes. Cross-sectional multicentre study carried out in Spanish community pharmacies during 4 weeks, one in each season of the year. Oral J01 antibiotics (Anatomical Therapeutic Chemical classification, ATC) requested for self-medication were analysed. 247 pharmacies and 365 pharmacists participated in the study, 1172 antibiotic requests were recorded. More frequent requests for antibiotic self-medication were previous treatment for the same symptoms (63.1%), insufficient time for a general practitioner (GP) consultation (12.1%) and patient belief that GP is going to prescribe the antibiotic (4.9%). Higher number of generic medication were requested compared to brand medication (41.1 versus 34.9%). Active ingredients commonly requested were amoxicillin (28.2%), amoxicillin-clavulanic (14.9%) and phosphomycin (21.8%) to treat upper respiratory related infections (35.1%), urinary infections (28.1%) and teeth infections (20.2%). 2406 pharmacists' interventions were carried out referring to the GP (40.8%), information for correct use of medication (31.3%), non-pharmacological treatment (15.7%), and recommendation of a different medication (6.9%) or a different product (5.3%). Nowadays higher number of active ingredients than brands are requested when selecting a treatment for an infection, mainly β-lactams and macrolides. Majority of self-medication tried to treat upper respiratory infections, urinary and teeth infections. Most frequent pharmacists' intervention was referring to the GP to obtain a correct diagnosis and treatment.Nowadays higher number of active ingredients than brands are requested when selecting a treatment for an infection, mainly β-lactams and macrolides. Majority of self-medication tried to treat upper respiratory infections, urinary and teeth infections. Most frequent pharmacists' intervention was referring to the GP to obtain a correct diagnosis and treatment.