Acinetobacter junii strain H11S-25, Nitratireductor sp. strain H11S-31 and Pseudomonas sp. strain H11S-28 were used at a density ratio of 9541 to construct high-efficiency oil-degrading consortium H. After a three-day biodegradation experiment, consortium H showed high degradation efficiencies of 74.2% and 65.7% for total alkanes and PAHs, respectively. The degradation efficiency of biomarkers such as pristane and high-molecular-weight polycyclic aromatic hydrocarbons (such as CHR) reached 84.5% and 80.48%, respectively. The findings of this study indicate that the microorganisms in the deep-sea hydrothermal area are potential resources for degrading petroleum hydrocarbons. Consortium H, which was artificially constructed, showed a highly efficient oil-degrading capacity and has significant application prospects in oil pollution bioremediation.As an analytic pipeline for quantitative imaging feature extraction and analysis, radiomics has grown rapidly in the past decade. On the other hand, recent advances in deep learning and transfer learning have shown significant potential in the quantitative medical imaging field, raising the research question of whether deep transfer learning features have predictive information in addition to radiomics features. In this study, using CT images from Pancreatic Ductal Adenocarcinoma (PDAC) patients recruited in two independent hospitals, we discovered most transfer learning features have weak linear relationships with radiomics features, suggesting a potential complementary relationship between these two feature sets. selleckchem We also tested the prognostic performance for overall survival using four feature fusion and reduction methods for combining radiomics and transfer learning features and compared the results with our proposed risk score-based feature fusion method. It was shown that the risk score-based feature fusion method significantly improves the prognosis performance for predicting overall survival in PDAC patients compared to other traditional feature reduction methods used in previous radiomics studies (40% increase in area under ROC curve (AUC) yielding AUC of 0.84).Parental care is costly, thus theory predicts that parents should avoid caring for unrelated offspring. However, alloparenting has been reported in many taxa because it may increase the caregiver mating success or offspring survival. We experimentally investigated the existence of allopaternal care in two glassfrog species, Hyalinobatrachium chirripoi and Centrolene peristicta, and discussed possible costs and benefits. Males mated with multiple females and cared for clutches, while continued to call. In the field, we randomly placed unrelated clutches in the territory of males already caring for their clutches and in the territory of non-attending males. Attending males adopted unrelated clutches, whereas non-attending males abandoned their territories. Once males adopted unrelated offspring, they cared for all clutches in a similar frequency and gained new clutches. Alloparenting was context-dependent, as only males already caring for their clutches adopted unrelated ones. We suggest that steroid hormonal levels might mediate the adoption of unrelated offspring by attending males. Additionally, our results suggest that males do not directly discriminate between related and unrelated offspring. Alloparenting has been widely investigated in different vertebrates, except for amphibians. Thus, our study sheds light on the roles of alloparenting for offspring survival and mating success in this group.Hirschsprung disease (HD) is a congenital disorder in the distal colon that is characterized by the absence of nerve ganglion cells in the diseased tissue. The primary treatment for HD is surgical intervention with resection of the aganglionic bowel. The accurate identification of the aganglionic segment depends on the histologic evaluation of multiple biopsies to determine the absence of ganglion cells in the tissue, which can be a time-consuming procedure. We investigate the feasibility of using a combination of label-free optical modalities, second harmonic generation (SHG); two-photon excitation autofluorescence (2PAF); and Raman spectroscopy (RS), to accurately locate and identify ganglion cells in murine intestinal tissue without the use of exogenous labels or dyes. We show that the image contrast provided by SHG and 2PAF signals allows for the visualization of the overall tissue morphology and localization of regions that may contain ganglion cells, while RS provides detailed multiplexed molecular information that can be used to accurately identify specific ganglion cells. Support vector machine, principal component analysis and linear discriminant analysis classification models were applied to the hyperspectral Raman data and showed that ganglion cells can be identified with a classification accuracy higher than 95%. Our findings suggest that a near real-time intraoperative histology method can be developed using these three optical modalities together that can aid pathologists and surgeons in rapid, accurate identification of ganglion cells to guide surgical decisions with minimal human intervention.The oligosaccharide required for asparagine (N)-linked glycosylation of proteins in the endoplasmic reticulum (ER) is donated by the glycolipid Glc3Man9GlcNAc2-PP-dolichol. Remarkably, whereas glycosylation occurs in the ER lumen, the initial steps of Glc3Man9GlcNAc2-PP-dolichol synthesis generate the lipid intermediate Man5GlcNAc2-PP-dolichol (M5-DLO) on the cytoplasmic side of the ER. Glycolipid assembly is completed only after M5-DLO is translocated to the luminal side. The membrane protein (M5-DLO scramblase) that mediates M5-DLO translocation across the ER membrane has not been identified, despite its importance for N-glycosylation. Building on our ability to recapitulate scramblase activity in proteoliposomes reconstituted with a crude mixture of ER membrane proteins, we developed a mass spectrometry-based 'activity correlation profiling' approach to identify scramblase candidates in the yeast Saccharomyces cerevisiae. Data curation prioritized six polytopic ER membrane proteins as scramblase candidates, but reconstitution-based assays and gene disruption in the protist Trypanosoma brucei revealed, unexpectedly, that none of these proteins is necessary for M5-DLO scramblase activity.