These results show that complementary nondiffusing biochemical signals can be linked into PEG-DA hydrogels simultaneously using 'Catcher-based ligation strategies, thereby inducing more nuanced cell-material interactions.Gibbsian composite-system thermodynamics is the framework governing the equilibrium of composite systems, including systems that at equilibrium have more than one value of pressure because of the action of surface tension, semipermeable membranes, or fields, and thus cannot be treated as simple systems. J. W. Gibbs's paper that lays out composite-system thermodynamics, "On the Equilibrium of Heterogeneous Substances", communicated in two parts in 1876 and 1878, is widely regarded as one of the most important pieces of scientific literature of its century. Many scientists adopted and stressed the importance of Gibbsian thermodynamics. In 1960, H. B. ERK inhibitor Callen wrote a textbook that made Gibbsian composite-system thermodynamics more accessible to thermodynamicists. However, Callen's book left out Gibbs's work on curved fluid interfaces and did not treat the complicated nonideal systems of interest to today's thermodynamicists. In this Feature Article, I have attempted to convey in a comprehensive manner the framewos as the curvature-induced depression of the eutectic temperature or the removal of azeotropes by nanoscale fluid interface curvature. Gibbsian composite-system thermodynamics has broad applications in biotechnology, nanostructured materials, surface textures and coatings, microfluidics, nanoscience, atmospheric and environmental physics, among others.Juvenile hormone (JH) is an important endocrine factor regulating many biological activities in arthropods. In daphnids, methoprene-tolerant (Met) belongs to a basic helix-loop-helix/Per-Arnt-Sim (bHLH/PAS) family protein which has recently been confirmed as a JH receptor and can bind and be activated by JHs and JH agonists. Although the activation of the JH signaling pathway causes many physiological effects, the molecular basis for the structural feature and ligand binding properties of Daphnia Met are not fully understood. To study the ligand preference in terms of structural features of Daphnia Met, we built in silico homology models of the PAS-B domain of Daphnia Mets from cladoceran crustaceans, Daphnia pulex and D. magna. Structural comparison of two Daphnia Met PAS-B domain models revealed that the volume in the main cavity of D. magna Met was larger than that of D. pulex Met. Compared with insect Met, Daphnia Met had a less hydrophobic cavity due to polar residues in the core-binding site. Molecular docking simulations of JH and its analogs with Daphnia Met indicated that the interaction energies were correlated with each of the experimental values of in vivo JH activities based on male induction and in vitro Met-mediated transactivation potencies. Furthermore, in silico site-directed mutagenesis supported experimental findings that Thr292 in D. pulex Met and Thr296 in D. magna Met substitution to valine contribute to JH selectivity and differential species response. This study demonstrates that in silico simulations of Daphnia Met and its ligands may be a tool for predicting the ligand profile and cross species sensitivity.Dimers of metallic nanowires (NWs) with nanometric gaps could be an alternative to overcome the limitations of existing plasmonic waveguides. The gap-surface plasmon polaritons (gap-SPPs) of the dimers may propagate along the NW without crosstalk and greatly enhance the coupling efficiency with an emitter, enabling ultracompact optical circuits. Such a possibility has not been realized, and we experimentally show its possibility. The gap-SPPs of the AgNW-molecule-AgNW structure, with a gap of 3-5 nm defined by the molecules, are visualized using the surface-enhanced Raman scattering (SERS) of the molecules. The SERS images, representing the gap-field intensity distribution, reveal the decay and beating of the monopole-monopole and dipole-dipole gap modes. The propagation lengths of the two (l1 = 0.5-2 μm and l2 = 5-8 μm) closely follow the model prediction with a uniform gap, confirming that the scattering loss induced by the gap irregularities is surprisingly low.This work presents the first use of yeast-displayed protein targets for screening mRNA-display libraries of cyclic and linear peptides. The WW domains of Yes-Associated Protein 1 (WW-YAP) and mitochondrial import receptor subunit TOM22 were adopted as protein targets. Yeast cells displaying WW-YAP or TOM22 were magnetized with iron oxide nanoparticles to enable the isolation of target-binding mRNA-peptide fusions. Equilibrium adsorption studies were conducted to estimate the binding affinity (KD) of select WW-YAP-binding peptides KD values of 37 and 4 μM were obtained for cyclo[M-AFRLC-K] and its linear cognate, and 40 and 3 μM for cyclo[M-LDFVNHRSRG-K] and its linear cognate, respectively. TOM22-binding peptide cyclo[M-PELNRAI-K] was conjugated to magnetic beads and incubated with yeast cells expressing TOM22 and luciferase. A luciferase-based assay showed a 4.5-fold higher binding of TOM22+ yeast compared to control cells. This work demonstrates that integrating mRNA- and yeast-display accelerates the discovery of peptide ligands.During the development of antibacterial and antiviral materials for personal protective equipment (PPE), daylight active functional polymeric materials containing vitamin K compounds (VKs) and impacts of polymer structures to the functions were investigated. As examples, hydrophobic polyacrylonitrile (PAN) and hydrophilic poly(vinyl alcohol-co-ethylene) (PVA-co-PE) polymers were directly blended with three VK compounds and electrospun into VK-containing nanofibrous membranes (VNFMs). The prepared VNFMs exhibited robust photoactivity in generating reactive oxygen species (ROS) under both daylight (D65, 300-800 nm) and ultraviolet A (UVA, 365 nm) irradiation, resulting in high antimicrobial and antiviral efficiency (>99.9%) within a short exposure time ( less then 90 min). Interestingly, the PVA-co-PE/VK3 VNFM showed higher ROS production rates and better biocidal functions than those of the PAN/VK3 VNFM under the same photoirradiation conditions, indicating that PVA-co-PE is a better matrix polymer material for these functions.