An important determinant of alcohol and tobacco use is the adolescent's social network, which has not been explored among out of school youth (OSY). OSY are adolescents not currently enrolled in school and have not completed their schooling. This study aims to qualitatively understand how OSY's social networks support or constrain alcohol and tobacco use. Respondent-driven sampling was used to select 41 OSY (aged 13-20 years) for individual in-depth interviews in a South African urban area. The data were analysed using content analysis. Smoking and drinking friends, family close in age to OSY that drank and smoked, and lack of parental support were associated with alcohol and tobacco use among OSY. Household norms, romantic partners and non-smoking or non-drinking friends were suggested to mitigate alcohol and tobacco use. Understanding how the social network of OSY plays a role in alcohol and tobacco use is useful for gaining an insight into the profile of OSY at risk for alcohol and tobacco use. Registration of OSY youth and community-based peer led programmes that include influential OSY family and friends could be beneficial.Global amphibian populations are being decimated by chytridiomycosis, a deadly skin infection caused by the fungal pathogens Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal). Although ongoing efforts are attempting to limit the spread of these infections, targeted treatments are necessary to manage the disease. Currently, no tools for genetic manipulation are available to identify and test specific drug targets in these fungi. To facilitate the development of genetic tools in Bd and Bsal, we have tested five commonly used antibiotics with available resistance genes Hygromycin, Blasticidin, Puromycin, Zeocin, and Neomycin. We have identified effective concentrations of each for selection in both liquid culture and on solid media. These concentrations are within the range of concentrations used for selecting genetically modified cells from a variety of other eukaryotic species.There is an old soccer wisdom that a goal scored just before halftime has greater value than other goals. Many dismiss this old wisdom as just another myth waiting to be busted. To test which is right we have analysed the final score difference through linear regression and outcome (win, draw, loss) through logistic regression. We use games from many leagues, control for the halftime score, comparing games in which a goal was scored after 1 minute remained of regulation time with games in which it was scored before the 44th minute. Our main finding is that the home team scoring just before halftime influence these outcomes to its advantage, compared with scoring earlier with the same halftime score. We conclude that a goal scored just before halftime has greater value than other goals provided it is scored by the home team. In other words; the wisdom may be old, but it's still wise.The robustness of networks against node failure and the response of networks to node removal has been studied extensively for networks such as transportation networks, power grids, and food webs. In many cases, a network's clustering coefficient was identified as a good indicator for network robustness. In ecology, habitat networks constitute a powerful tool to represent metapopulations or -communities, where nodes represent habitat patches and links indicate how these are connected. Current climate and land-use changes result in decline of habitat area and its connectivity and are thus the main drivers for the ongoing biodiversity loss. Conservation efforts are therefore needed to improve the connectivity and mitigate effects of habitat loss. Habitat loss can easily be modelled with the help of habitat networks and the question arises how to modify networks to obtain higher robustness. Here, we develop tools to identify which links should be added to a network to increase the robustness. We introduce two different heuristics, Greedy and Lazy Greedy, to maximize the clustering coefficient if multiple links can be added. We test these approaches and compare the results to the optimal solution for different generic networks including a variety of standard networks as well as spatially explicit landscape based habitat networks. In a last step, we simulate the robustness of habitat networks before and after adding multiple links and investigate the increase in robustness depending on both the number of added links and the heuristic used. We found that using our heuristics to add links to sparse networks such as habitat networks has a greater impact on the clustering coefficient compared to randomly adding links. The Greedy algorithm delivered optimal results in almost all cases when adding two links to the network. Furthermore, the robustness of networks increased with the number of additional links added using the Greedy or Lazy Greedy algorithm.The purpose of this study was to assess the effects of three different programs, i.e. active dynamic warm-up program plus functional resistance warm-up using Theraband plus pre-shot routine program (AFPR); pre-shot routine program (PR); and active dynamic warm-up program plus functional resistance warm-up using Theraband (AF) on driver club head speed, driving distance, and driving accuracy in the amateur golfers. Fifteen amateur golfers with an average age of 19.67 ± 0.89 years and 4.87 ± 1.77 points of average handicap were assigned to participate in either AFPR, PR or AF program. All participants in the three programs practiced three sessions on non-consecutive days per week during the intervention phase. Each participant's performance was assessed before and after six weeks of the program through hitting ten maximal drives with the ball flight and swing analyzed using the P3ProSwing Golf Simulator and recorded for the driver club head speed, driving distance, and driving accuracy. Multivariate analysis of variance showed no statistically significant differences (P less then .05) of the performances of the golfers participated in the 3 programs (club head speed F = 1.02, P = 0.33; accuracy F = 0.32, P = 0.72; distance F = 0.18, P = 0.83). Furthermore, a paired t-tests also showed no statistically significant (P less then .05) improvement occurred in the 3 programs after the six-week training. ATX968 Although the effect of the 3 programs did not show statistically significant increase in the performance of the amateur golfers, however, the three parameters of the performance, i.e. the driver club head speed, the driving distance and the driving accuracy showed certain improvements. The 3 training programs may have benefit to the amateur golfers with certain increases of their performance.