Our study further showed that the small-molecule drug CMA/BAF can overcome drug resistance by disrupting the interaction between KCNJ15 and the lysosomal system. In summary, KCNJ15's role in breast cancer drug resistance and survival warrants consideration in the selection of treatment approaches.In the realm of adult brain tumors, Glioblastoma stands out as the most aggressive type. Still, the effectiveness of the current standard treatment protocol is negatively affected by the problem of drug resistance and the suppressive immune microenvironment. The recently discovered form of cell death, ferroptosis, reliant on iron, shows excellent prospects as a chemosensitizer. Erastin, an inducer of ferropotosis, can substantially enhance the sensitivity of glioblastoma cells to Temozolomide chemotherapy, leading to improved anti-tumor activity. A hydrogel-liposome nanoplatform containing both Temozolomide and Erastin, an inducer of ferroptosis, was engineered as part of this study. To target glioblastomas, the codelivery system was modified using the v3 integrin-binding peptide, cyclic RGD. In their capacity as biocompatible drug reservoirs, cross-linked GelMA (gelatin methacrylamide) hydrogel and cRGD-coated liposomes successfully achieved a sustained release of their internal contents. The GelMA-liposome system, utilized in a modified intracranial tumor resection model, achieved a prolonged slow-release of Temozolomide and Erastin in situ, lasting beyond 14 days. Nanoplatform (T+E@LPs-cRGD+GelMA) enhancement of glioblastoma sensitivity to temozolomide chemotherapy resulted in satisfactory anti-tumor outcomes, as indicated by the results. Therapeutic strategies centered around the induction of ferroptosis proved effective in overcoming drug resistance. Subsequently, transcriptome sequencing was carried out to determine the fundamental mechanism responsible for the nanoplatform (T+E@LPs-cRGD+GelMA)'s effect. It is considered that the GelMA-liposome system plays a potential role in the immune response and immunomodulation of glioblastoma, through the interferon/PD-L1 pathway. Through a collective analysis, this study offered a possible combination therapy for managing glioblastoma.Because of their inherent biocompatibility and biodegradability, liposomes stand out as a frequently employed drug carrier. The complex nature of formulation components and the elaborate preparation procedure usually compel researchers to adopt a trial-and-error approach to screening, which is frequently marked by low effectiveness. Through the use of machine learning (ML), prediction models for liposome formulations have been built. Key liposome properties—size, polydispersity index (PDI), zeta potential, and encapsulation—are independently predicted by an optimal machine learning algorithm, while formulation factors are ranked, offering useful insights to guide formulation design. Reviewing key parameters, drug molecules with logS values between -3 and -6, molecular complexities between 500 and 1000, and XLogP3 of 2 show high potential for enhanced liposome preparation and encapsulation. ly3295668 inhibitor Liposome formulations were developed for naproxen (NAP), an insoluble molecule, and palmatine HCl (PAL), a water-soluble molecule, to ascertain the model's predictive capabilities. The alignment of predicted and experimental results underscores the satisfactory accuracy of machine learning models. To better understand the molecular interactions and dynamics within NAP and PAL liposomes, coarse-grained molecular dynamics simulations are employed, given the critical role of drug properties in liposome particles. The model's architecture reveals that NAP molecules have the potential to be disseminated within the lipid layer, whereas PAL molecules are largely concentrated within the interior aqueous portion of the liposome. The contrasting physical states of NAP and PAL highlight the crucial role of drug characteristics in shaping liposome formulations. In conclusion, these models for predicting liposome formulations are constructed and the impacts of essential factors are analyzed through a fusion of molecular modeling and machine learning approaches. The findings of this study affirm the availability and rational foundation of these intelligent prediction systems, potentially enabling their future application in the creation of liposome formulations.Mesenchymal stem cells (MSCs), possessing immunomodulatory, anti-inflammatory, and regenerative repair functions, stand out for their potency in cartilage regeneration. Meanwhile, the critical factors propelling further utilization of these drugs have been their intra-articular delivery mechanisms and their synergistic interactions with other therapeutic agents. A multifunctional hydrogel system, bio-inspired by mussels, is presented for the co-delivery and synergistic effect of MSC-derived exosomes (Exos) with icariin (ICA). Cartilage regeneration is anticipated to result from the ICA and Exos co-delivered articular cavity injection system's sustained presence within the joint cavity. This is possible due to the thermosensitive, self-healing, and adhesive characteristics of the mussel-inspired multifunctional hydrogel. Exos, as evidenced by the experimental results, increased the cellular uptake of ICA by more than double, and this synergy between Exos and ICA substantially improved cell proliferation and migration rates. Synergistic treatment led to a reduction of matrix metalloproteinase 13 in the supernatant by 47%, and a decrease of 59% within the intracellular space. Through multifunctional hydrogel delivery, in vivo studies demonstrated the prolonged retention of ICA-loaded Exos, consequently improving cartilage protection. Hydrogel co-delivery systems, in osteoarthritis models, successfully reversed cartilage loss, thereby maintaining adequate cartilage thickness.Biopolymers, possessing a benign environmental profile, demonstrate applicability across numerous sectors. These materials' exceptional characteristics, encompassing bioactivity, renewability, bioresorbability, biocompatibility, biodegradability, and hydrophilicity, render them highly favorable for use in implantable biomedical devices. Additive manufacturing (AM) stands as a versatile and intricate fabrication method, extensively employed in the creation of tailored biopolymer products and structures for state-of-the-art healthcare applications. In functional clinical settings, the 3D printing of sustainable materials is employed for diverse purposes, including wound dressings, drug delivery systems, the creation of medical implants, and tissue engineering. The present review summarizes recent developments in various biopolymer types, proteins and polysaccharides in particular. These substances are integral to the creation of numerous biomedical products via extrusion, vat polymerization, laser and inkjet 3D printing, along with fundamental bioprinting, and four-dimensional (4D) bioprinting techniques. Incorporating the effects of nanoparticles, this review analyzes the biological and mechanical performance of 3D-printed tissue scaffolds. This work tackles current environmental concerns and future advancements in AM-manufactured, eco-friendly polymeric materials. More focused research is essential to optimally blend these biodegradable biopolymers and produce impactful outcomes in targeted biomedical applications. The biomedical sector is poised for significant transformation thanks to the potential of biopolymer-based 3D-printed composites.The unfortunate truth surrounding acute liver injury (ALI) is its elevated fatality rate, stemming from ineffective and untimely treatment approaches. Schisandrin B (SchB), although extensively used for treating diverse liver conditions, exhibited limited therapeutic efficacy on acute lung injury (ALI) owing to its high hydrophobicity. Serum albumin modified with palmitic acid (PSA) serves not only as an efficient carrier for hydrophobic medications but also provides superior targeting capabilities for M1 macrophages via scavenger receptor-A (SR-A), potentially revolutionizing ALI treatment. PSA's drug delivery system, unlike typical macrophage-based approaches, allows for precise targeting to reduce adverse effects outside the desired area. SchB-PSA nanoparticles were synthesized and their therapeutic impact on ALI was further investigated. In vitro, SchB-PSA nanoparticles showcased enhanced cytotoxicity against lipopolysaccharide-stimulated Raw2647 (LAR) cells, compared to SchB-HSA nanoparticles. A striking observation was the 879-fold higher uptake of PSA nanoparticles by LAR cells than that of HSA nanoparticles. In keeping with the expectation, there was a higher level of PSA NP accumulation in the liver. Significantly, SchB-PSA NPs dramatically lowered the activation of NF-κB signaling, effectively reducing inflammatory response and hepatic necrosis to a considerable degree. The high dose of SchB-PSA NPs demonstrably enhanced the survival rate in ALI mice to a remarkable 75% in the 72-hour time frame. Therefore, SchB-PSA NPs show great potential for treating ALI.This nanomaterial's creation through microbial processes constitutes a novel research technique. A culture medium served as the environment where Trichophyton rubrum biosynthesized silver nanoparticles. The generation of silver nanoparticles was facilitated in this study by the utilization of Trichophyton rubrum, a dermatophyte fungus. A mineral salt medium served as the culture environment for the clinical strains of these species, which were incubated at 25°C for a period spanning 5 to 7 days. To synthesize AgNps, each culture's cell-free filtrate was treated with a 1 mM AgNO3 solution. The solution's color transition to reddish-light brown after 72 hours provided virtual evidence of the Ag+ ion reduction process in metal nanoparticles. To detect AgNo3, SEM was employed. AgNPs, primarily spherical and measuring 100 nanometers in diameter, were identified using SEM. Moreover, the research revealed that silver nanoparticles exhibited antifungal properties against both infections, demonstrating a correlation with concentration levels. Significant deceleration in growth was witnessed with an AgNPs concentration of 150 ppm.