The choice of suitable solvent with satisfactory viscosity, miscibility and biocompatibility along with considerable PLGA hydrophobicity and molecular weights is fundamental for optimizing the drug release. This overview gives a particular emphasis on evaluations and the wide ranges of requirements needed to achieve reasonable physicochemical characteristics of ISFIs.Prenylated (iso)flavonoid-type compounds are a subclass of natural flavonoids that have been reported to exhibit good antioxidant properties. In the present paper, the structure-activity relationship of three typical prenylated (iso)flavonoids namely 8-prenyldaidzein (Per), Licoflavone (Lic), and erysubin F (Ery) have been determined using DFT (density functional theory)-based calculations and molecular docking studies. As result, the CH bond of the prenyl substituent was found to be the most thermodynamically favorable site for trapping free radicals in the gas phase and lipid physiological environments. While the OH bond of the B-ring seems to be more reactive in water. HAT (hydrogen atom transfer) and SPLET (sequential proton loss electron transfer) play a decisive role in the antiradical activity of the studied compounds in lipid and polar physiological environments, respectively. All of the studied compounds exhibit strong binding affinity to both xanthine oxidase and inducible nitric oxide synthase enzymes by forming several hydrogen bonds and hydrophobic interactions with their respective catalytic sites. These results suggest that (iso)flavonoid-type compounds are promising radical scavengers and antioxidants.Communicated by Ramaswamy H. Sarma.This study describes asymmetry in key mechanical variables during a treadmill-based, running graded exercise test (GXT). Twenty-one recreationally trained male runners completed a continuous, maximal GXT on an instrumented treadmill, starting at 9 km.h-1 with speed increases of +0.5 km.h-1 every 30 s, for the determination of ventilatory threshold (VT), respiratory compensation point (RCP), and maximal oxygen uptake (MAX). Ground reaction forces were recorded continuously and subsequently averaged from 10 consecutive steps corresponding to VT, RCP and MAX intensity stages (13.4 ± 1.2 km.h-1, 16.0 ± 1.6 km.h-1 and 18.2 ± 1.5 km.h-1, respectively). Asymmetry scores were assessed from the "symmetry angle" (SA) formulae, where a score of 0%/100% indicates perfect symmetry/asymmetry; these were then compared between the three intensity stages. There was no influence of exercise intensity on SA scores for any of the sixteen biomechanical variables (P > 0.222). The group mean SA scores did not exceed 1.5% for spatio-temporal variables (contact time, aerial time, frequency and step length). There were larger mean SA scores for mean loading rate (3.7 ± 2.7%) and most spring-mass model variables (vertical stiffness 2.2 ± 1.6% and leg stiffness 1.7 ± 1.4%). The SA scores were ∼1.0-3.5% for braking and propulsive phase durations, peak forces, and resulting impulses. Lower extremities behave similarly at submaximal and maximal intensities during GXT, indicating that runners maintained relatively even strides as intensity increased. However, practitioners must be careful not to infer the presence of asymmetry during GXT based on a single variable, given the lower SA scores for spatio-temporal parameters.Combining myocardial perfusion single photon emission computed tomography (SPECT-MPI) and coronary computed tomography angiography (CCTA) is an interesting hybrid imaging option in modern cardiovascular medicine. The integrated hybrid technique has a number of advantages compared to visual side-by-side analysis of the separate modalities. CT attenuation map can correct for attenuation artefacts and thus improve the diagnostic accuracy of SPECT-MPI. Moreover, the anatomical information of the CCTA and the perfusion map of SPECT-MPI allow for vessel-based correlation and culprit vessel identification. Combining SPECT-MPI with CCTA is an appealing tool in the work-up of complex ischaemic heart disease and might help determine the optimal treatment strategy. This case series illustrates the role of SPECT-CCTA in decision-making of revascularization strategy in complex ischaemic heart disease.In South Africa, Candida auris was the third most common cause of candidemia in 2016-2017. We performed single nucleotide polymorphism (SNP) genome-wide analysis of 115 C. auris isolates collected between 2009 and 2018 from national laboratory-based surveillance, an environmental survey at four hospitals and a colonization study during a neonatal unit outbreak. The first known South African C. auris strain from 2009 clustered in clade IV. Overall, 98 strains clustered within clade III (85%), 14 within clade I (12%) and three within clade IV (3%). All environmental and colonizing strains clustered in clade III. YAP-TEAD Inhibitor 1 We also identified known clade-specific resistance mutations in the ERG11 and FKS1 genes. Identification of clade I strains between 2016 and 2018 suggests introductions from South Asia followed by local transmission. SNP analysis characterized most C. auris strains into clade III, the clade first reported from South Africa, but the presence of clades I and IV strains also suggest early introductions from other regions. Sheng Xue Fang (SXF) has been used to treat anaemia for decades with good efficacy. To study the effect and possible mechanism of SXF to restore haematopoietic function. Balb/c mice (10 per/group, half male, half female) were treated with SXF (three dose groups, 8.5, 17, and 22.1 g/kg) by gavage for 14 days, and cyclophosphamide (80 mg/kg) was injected on days 10-12. Only injection of cyclophosphamide (negative control) or physiological saline (blank control) were included as controls. The spleen and femur were processed for histopathology. Active components and the target of SXF were screened. The target was used for gene enrichment and protein-protein interaction (PPI) analysis. Red blood cell relative changes in the SXF group (low -5.50 ± 1.58%; medium -11.11 ± 4.15%; high -8.81 ± 2.67%) and relative negative control (26.21 ± 2.51%) significantly increased (all  < 0.01) in female mice. Haemoglobin and red blood cell-specific volume showed the same trend. However, SXF did not have significant effects on male mice.