3 mg/mL. Taken together, our results demonstrate that A. fruticosa fruits EO contains active compounds with selective inhibitory effect on different microbial strains in planktonic and biofilm growth state, explained at least partially by the interference with microbial membranes due to their hydrophobic character.The goal of the work was to develop materials dedicated to spine surgery that minimized the potential for infection originating from the transfer of bacteria during long surgeries. The bacteria form biofilms, causing implant loosening, pain and finally, a risk of paralysis for patients. Our strategy focused both on improvement of antibacterial properties against bacteria adhesion and on wear and corrosion resistance of tools for spine surgery. Further, a ~35% decrease in implant and tool dimensions was expected by introducing ultrahigh-strength titanium alloys for less-invasive surgeries. The tested materials, in the form of thin, multi-layered coatings, showed nanocrystalline microstructures. Performed direct-cytotoxicity studies (including lactate dehydrogenase activity measurement) showed that there was a low probability of adverse effects on surrounding SAOS-2 (Homo sapiens bone osteosarcoma) cells. The microbiological studies (e.g., ISO 22196 contact tests) showed that implanting Ag nanoparticles into Ti/TixN coatings inhibited the growth of E. coli and S. aureus cells and reduced their adhesion to the material surface. These findings suggest that Ag-nanoparticles present in implant coatings may potentially minimize infection risk and lower inherent stress. Erythritol, a sugar alcohol, is widely used as a substitute for sugar in diets for patients with diabetes or obesity. In this study, we aimed to investigate the effects of erythritol on metabolic disorders induced by a high-fat diet in C57BL/6J mice, while focusing on changes in innate immunity. Mice that were fed a high-fat diet and administered water containing 5% erythritol (Ery group) had markedly lower body weight, improved glucose tolerance, and markedly higher energy expenditure than the control mice (Ctrl group) ( = 6). Furthermore, compared with the Ctrl group, the Ery group had lesser fat deposition in the liver, smaller adipocytes, and significantly better inflammatory findings in the small intestine. The concentrations of short-chain fatty acids (SCFAs), such as acetic acid, propanoic acid, and butanoic acid, in the serum, feces, and white adipose tissue of the Ery group were markedly higher than those in the Ctrl group. In flow cytometry experiments, group 3 innate lymphoid cell (ILC3) counts in the lamina propria of the small intestine and ILC2 counts in the white adipose tissue of the Ery group were markedly higher than those in the Ctrl group. Quantitative real-time reverse transcription polymerase chain reaction analyses showed that the Il-22 expression in the small intestine of the Ery group was markedly higher than that in the Ctrl group. Erythritol markedly decreased metabolic disorders such as diet-induced obesity, glucose intolerance, dyslipidemia, and fat accumulation in the mouse liver by increasing SCFAs and modulating innate immunity.Erythritol markedly decreased metabolic disorders such as diet-induced obesity, glucose intolerance, dyslipidemia, and fat accumulation in the mouse liver by increasing SCFAs and modulating innate immunity.Modern sensors deployed in most Industry 4.0 applications are intelligent, meaning that they present sophisticated behavior, usually due to embedded software, and network connectivity capabilities. For that reason, the task of calibrating an intelligent sensor currently involves more than measuring physical quantities. TAK-228 As the behavior of modern sensors depends on embedded software, comprehensive assessments of such sensors necessarily demands the analysis of their embedded software. On the other hand, interlaboratory comparisons are comparative analyses of a body of labs involved in such assessments. While interlaboratory comparison is a well-established practice in fields related to physical, chemical and biological sciences, it is a recent challenge for software assessment. Establishing quantitative metrics to compare the performance of software analysis and testing accredited labs is no trivial task. Software is intangible and its requirements accommodate some ambiguity, inconsistency or information loss. Besides, software testing and analysis are highly human-dependent activities. In the present work, we investigate whether performing interlaboratory comparisons for software assessment by using quantitative performance measurement is feasible. The proposal was to evaluate the competence in software code analysis activities of each lab by using two quantitative metrics (code coverage and mutation score). Our results demonstrate the feasibility of establishing quantitative comparisons among software analysis and testing accredited laboratories. One of these rounds was registered as formal proficiency testing in the database-the first registered proficiency testing focused on code analysis.The machining of Ti6Al4V alloy, especially at low cutting speeds, is associated with strong Built-Up Edge (BUE) formation. The PVD coatings applied on cutting tools to machine such materials must have the necessary combination of properties to address such an underlying wear mechanism. The present work investigates and shows that TiB2 PVD coating can be designed to have certain mechanical properties and tribological characteristics that improve machining in cases where BUE formation is observed. Three TiB2 coatings were studied one low hardness coating and two high hardness coatings with varied coating thicknesses. Wear performances for the various TiB2 coated carbide tools were evaluated while rough turning Ti6Al4V. Tool wear characteristics were evaluated using tool life studies and the 3D wear volume measurements of the worn surface. Chip morphology analyses were done to assess the in-situ tribological performance of the coatings. The micro-mechanical properties of the coatings were also studied in detail to co-relate with the coatings' performances.