The enzymatic efficiency (Kcat/Km) is highest for Ang V and Ang III followed by Ang IV and is lowest for Ang II. learn more As a gluzincin metallopeptidase, ACE2 requires a zinc molecule at its active site for catalysis. This report also documents inhibition of ACE2 activity by concentrations of zinc exceeding 10 μM. These observations extend the functional significance of ACE2 to include the metabolic inactivation of Ang III, Ang IV and Ang V, reemphasizing the importance of monitoring zinc intake to maintain metabolic homeostasis.In chalcocite (Cu2S) bioleaching, the lack of iron metabolism is a key restricting factor. As the most common sulfide mineral, pyrite (FeS2) can release Fe(Ⅱ) and compensate for the iron metabolism deficiency in chalcocite bioleaching. The bioleaching of chalcocite in an imitated industrial system was improved by enhancing the iron-sulfur metabolism simultaneously using pyrite and sulfur oxidizers based on the joint utilization of waste resources, while the bioleaching performance and community structure in the leachate were systematically investigated. Due to the active sulfur/iron metabolism, the pH reached 1.2, and Fe3+ was increased by 77.78%, while the biomass of planktonic cells was improved to 2.19 × 107 cells/mL. Fourier transform infrared reflection (FTIR) and X-ray diffraction (XRD) analysis results showed that more iron-sulfur crystals were produced due to more active iron-sulfur metabolism. Scanning electron microscopy (SEM) revealed that many derivative particles and corrosion marks appeared on the surface of the ore, implying that the mineral-microbe interaction was strengthened. Confocal laser scanning microscopy (CLSM) showed the accumulation of cells and extracellular polymeric substances (EPS) on the ore surface, indicating a stronger contact leaching mechanism. Furthermore, the community structure and canonical correspondence analysis (CCA) demonstrated that the introduction of sulfur-oxidizing bacteria and pyrite could maintain the diversity of dominant leaching microorganisms at a high level. Sulfobacillus (27.75%) and Leptospirllillum (20.26%) were the dominant sulfur-oxidizing and iron-oxidizing bacteria during the bioleaching process. With the accumulation of multiple positive effects, the copper ion leaching rate was improved by 44.8%. In general, this new type of multiple intervention strategy can provide an important guide for the bioleaching of low-grade ores.Antibiotic resistance is a global health emergency linked to unrestrained use of pharmaceutical and personal care products (PPCPs) as prophylactic agent and therapeutic purposes across various industries. Occurrence of pharmaceuticals are identified in ground water, surface water, soils, and wastewater treatment plants (WWTPs) in ng/L to μg/L concentration range. The prevalence of organic compounds including antimicrobial agents, hormones, antibiotics, preservatives, disinfectants, synthetic musks etc. in environment have posed serious health concerns. The aim of this review is to elucidate the major sources accountable for emergence of antibiotic resistance. For this purpose, variety of introductory sources and fate of PPCPs in aquatic environment including human and veterinary wastes, aquaculture and agriculture related wastes, and other anthropogenic activities have been discussed. Furthermore, genetic and enzymatic factors responsible for transfer and appearance of antibiotic resistance genes are presented. Ecotoxicity of PPCPs has been studied in environment in order to present risk imposed to human and ecological health. As per published literature reports, the removal of antibiotics and related traces being difficult, couples the possibility of emergence of antibiotic resistance and hence sustainability in global water resources. Therefore, research on environmental behavior and control strategies should be conducted along with assessing their chronic toxicity to identify potential human and ecological risks.The discovery of cheap and eco-friendly functional materials for the removal of anionic heavy metal ions is still challenging in the treatment of heavy metal-contaminated water. Herein, a new poly(allyltrimethylammonium) grafted chitosan and biochar composite (PATMAC-CTS-BC) was introduced for the removal of selenate (SeO42-) in water. Results suggest that the PATMAC-CTS-BC showed a rapid removal of SeO42- with efficiency of >97% within 10 min and it followed a pseudo-second-order model. High capacity of SeO42- adsorption by the composite was achieved, with maximum value of 98.99 mg g-1 based on Langmuir model, considerably higher than most of reported adsorbents. The thermodynamic results reflected the spontaneous and exothermic nature of SeO42- adsorption onto the composite. The composite could be applied at a wide initial pH range (2-10) with high removal efficiency of SeO42- because of permanent positive charges of quaternary ammonium groups (=N+-). The removal mechanisms of SeO42- were mainly attributed to electrostatic interactions with =N+- and protonated -NH3+ groups, and redox-complexation interactions with -NH2, -NH-, and -OH groups. Besides SeO42-, the hexavalent chromium (Cr2O72-) was considered as example to further demonstrate the anion removal capability of cationic hydrogel-BC composite. The study outcomes open up new opportunities to efficiently remove anionic heavy metal ions (e.g., SeO42- and Cr2O72-) in water using these materials.The bioaccumulation of hexavalent uranium (U(VI)) on Candida utilis (C. utilis) and its biological effects were investigated via batch and biologic techniques. The bioaccumulation mechanism of U(VI) and C. utilis were characterized by SEM, TEM, FT-IR and XPS. The batch results showed that C. utilis had a high adsorption capacity (41.15 mg/g wet cells at pH 5.0) and high equilibrium rate (~100% within 3.5 h). The analysis of intracellular hydrogen peroxides and malondialdehyde suggested that the growth of C. utilis was inhibited under different concentrations of U(VI) due to the abundant production of reactive oxide species. The activity of intracellular antioxidants (e.g., super oxide dismutase and glutathione) was significantly enhanced under U(VI) stress, indicating the anti-toxic effect of C. utilis cells under low U(VI) stress. These results indicated that C. utilis is an ideal biosorbent for removing radionuclides in environmental remediation.