hairlead2
hairlead2
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Arochukwu, Plateau, Nigeria
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In the summer of 2018, specimens of a Hoplolaimus population were extracted from a maize root sample collected near Stampriet, Namibia. This population was identified as Hoplolaimus pararobustus and is described and illustrated based on its morphological, morphometric, and molecular characteristics. To our knowledge, this is the first report H. pararobustus from maize roots. Females of the population had a mean body and stylet length of 1,100 µm and 36 µm, respectively. Esophagus with three nuclei in three pharyngeal glands. Lateral field reduced, ranging from a very faint line to just breaks in striae. The males were shorter than the females with a mean body length of 925 µm and the stylet slightly shorter, with a mean length of 34 µm. Phylogenetic analyses using partial sequences of 18 S and the expansion fragment D2-D3 of 28 S rDNA genes showed the close relation of this species and H. columbus. This Namibian population of H. pararobustus is the first Hoplolaimus species from Africa to be molecularly characterized.The objective of this study was to evaluate the ability of an unmanned aerial system (UAS) equipped with a multispectral sensor to track plant health in the presence of plant-parasitic nematodes in conjunction with nematicide applications. Four nematicides were evaluated for their ability to suppress Belonolaimus longicaudatus and Meloidogyne incognita in microplots, and three nematicides were evaluated on a golf course for their ability to suppress multiple plant-parasitic nematode genera. Visual ratings, Normalized Difference Vegetation Index (NDVI), and Normalized Difference RedEdge Index (NDRE) were reported throughout the trial to assess plant health. B. longicaudatus and M. incognita population density was significantly lowered by nematicide treatments in microplots and correlated with visual ratings, NDVI, and NDRE plant health ratings. On the golf course, all nematicides reduced total plant-parasitic nematode population density at 28, 56, and 84 days after treatment (DAT). Visual turf quality ratings, NDVI, and NDRE were positively correlated with lower nematode population density in the majority of evaluation dates. In the microplot and golf course settings, the parameters evaluated for plant health were correlated with plant-parasitic nematode population density visual ratings, NDVI, and NDRE improved as nematode population density declined. These results show that remote sensing has the potential to be a beneficial tool for assessing plant-parasitic nematode infected bermudagrass.In Colombia, identification of entomopathogenic nematodes (EPN's) native species is of great importance for pest management programs. The aim of this study was to isolate and identify EPNs and their bacterial symbiont in the department of Cauca-Colombia and then evaluate the susceptibility of two Hass avocado (Persea americana) pests to the EPNs isolated. EPNs were isolated from soil samples by the insect baiting technique. Their bacterial symbiont was isolated from hemolymph of infected Galleria mellonella larvae. Both organisms were molecularly identified. Morphological, and biochemical characterization was done for the bacteria. Susceptibility of Epitrix cucumeris and Pandeleteius cinereus adults was evaluated by individually exposing adults to 50 infective juveniles. EPNs were allegedly detected at two sampled sites (natural forest and coffee cultivation) in 5.8% of the samples analyzed. However, only natural forest EPN's could be isolated and multiplied. The isolate was identified as Steinernema carpocapsae BPS and its bacterial symbiont as Xenorhabus nematophila BPS. YM155 Survivin inhibitor Adults of both pests were susceptible to S. carpocapsae indicating this EPN potential for its management. The results of this study constitute the first record of S. carpocapsae in Colombia and the susceptibility of P. cinereus to this EPN.The Okanagan Valley of British Columbia hosts a wine grape industry that has grown substantially in the past three decades in terms of both acreage and economic benefit to the region. The ring nematode, Mesocriconema xenoplax, has recently been found to be widespread in vineyard soils in the region. This study used field microplots to assess the potential impacts of a local population of M. xenoplax on the first four years growth of either self-rooted 'Merlot' or 'Merlot' vines grafted onto three commonly used rootstocks 3309C, 44-53M, and Riparia Gloire. The population of M. xenoplax multiplied to comparable levels on self-rooted vines and all rootstocks, indicating that none of the vine genotypes were resistant to M. xenoplax. Inoculation with M. xenoplax reduced cumulative pruning weights of self-rooted vines by 58%. Inoculation with M. xenoplax reduced trunk cross-sectional areas of 3309C by 45% and that of self-rooted vines by 38%, whereas it did not affect trunk cross-sectional areas of 44-53 or Riparia Gloire, indicating differing levels of rootstock tolerance to M. xenoplax. Our data suggest that M. xenoplax is likely impacting vineyard health and productivity in the region, and the selection of rootstocks and management practices to minimize impacts of this nematode should be considered in future vineyard replant management programs.We found that Nanidorus spp. was pathogenic to seashore paspalum (Paspalum vaginatum) turfgrass as its population increased from 100 to 2,080 nematodes per pot 180 days after inoculation under greenhouse conditions. Morphological measurements of adult females were similar to those described for N. minor. Molecular analysis also confirmed the morphological identification by targeting three different regions of the genomic DNA. Three primer pairs targeting 18S rDNA (360F/932R), 28S rDNA (D2A/D3B) and ITS1 rDNA (BL18/5818) were used in singleplex PCR. Forward and reverse sequences of each individual primer set were then subjected to multiple alignment and the complimentary sequences were assembled into a consensus sequence. Upon nucleotide blast on the NCBI website, they were all confirmed to be N. minor. A one-step multiplex PCR method using specific primers and a fragment size of 190 bp also confirmed the identity of N. minor. To the best of our knowledge, this is the first report of N. minor infecting seashore paspalum turfgrass in Georgia.

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