Dielectrophoresis (DEP) is an excellent tool for manipulating small particles within a liquid or gas medium. However, when the size of the particles is too small, such as with quantum dots (QDs), it is difficult to manipulate the particles using DEP because the dielectrophoretic force (FDEP) depends on the volume of the particles and is therefore too weak to achieve particle migration. Herein, we demonstrate a novel method for controlling nanoscale QD particles using DEP by introducing photopolymerized reactive mesogen (RM) bead vehicles. The size of an RM bead is well-controlled by the RM concentration in the medium, and when the size is approximately 0.2 μm or larger, the RM beads can be arbitrarily manipulated using DEP under moderate electric fields. Interestingly, during photopolymerization, QD particles are easily absorbed by polymerized RM beads and most of the QDs are embedded within the RM beads. Hence, we can fabricate periodic QD arrays by manipulating the RM beads containing such dots. In addition, we can fabricate multicolor QD arrays by repeating the processes using different QD particles. The shape of a DEP-assisted QD-RM network pattern can be precisely predicted by calculating the gradient of the square of the electric field (∇E2) and the corresponding FDEP. This new technology may be useful for the fabrication of optical devices, displays, photonic crystal devices, and bioapplications.The hydrazine group serves as a great anchor for bioconjugation; however, the application of hydrazone ligation has been limited by poor product stability. We aim to resolve such issues by optimizing the recently established pyrazolone ligation and investigating a new pyrazole ligation. We have identified a new, electron-deficient pyrazolone ligation and a regiospecific pyrazole ligation, both offering aqueous buffer stable and chemically inert products possessing triazole-like structures while not involving any heavy metal catalyst.Doxorubicin (DOX) is a cancer drug that binds to dsDNA through intercalation. A comprehensive microsecond timescale molecular dynamics study is performed for DOX with 16 tetradecamer dsDNA sequences in explicit aqueous solvent, in order to investigate the intercalation process at both binding stages (conformational change and insertion binding stages). The molecular mechanics generalized Born surface area (MM-GBSA) method is adapted to quantify and break down the binding free energy (BFE) into its thermodynamic components, for a variety of different solution conditions as well as different DNA sequences. Our results show that the van der Waals interaction provides the largest contribution to the BFE at each stage of binding. The sequence selectivity depends mainly on the base pairs located downstream from the DOX intercalation site, with a preference for (AT)2 or (TA)2 driven by the favorable electrostatic and/or van der Waals interactions. Invoking the quartet sequence model proved to be most successful to predict the sequence selectivity. Our findings also indicate that the aqueous bathing solution (i.e., water and ions) opposes the formation of the DOX-DNA complex at every binding stage, thus implying that the complexation process preferably occurs at low ionic strength and is crucially dependent on solvent effects.Ion mobility spectrometers (IMS) with field switching ion shutters are an excellent choice for trace gas detection, being extremely sensitive while having fast response times. However, as different target molecules may form positive, negative, or even ions of both polarities, it is beneficial to simultaneously detect both ion polarities. Here, we present a dual drift tube IMS with a new dual field switching ion shutter for gating both ion polarities and an X-ray ionization source in orthogonal configuration. The dual field switching ion shutter allows significantly improved ion gating and ion accumulation due to improved shielding of the ionization region from the drift field. Equipped with two 75 mm long high-performance drift tubes, the dual IMS reaches high resolving power of R = 90 with detection limits in the lower pptv range for different ketones, chlorinated hydrocarbons and methyl salicylate that forms ions in both polarities.Electrochemical aptamer-based (E-AB) biosensors suffer from sensor-to-sensor signal variations due to the variation of the total number and the heterogeneity of probes immobilized on the electrode surface, with the former attracting more attention. As such, a calibration process to correct for such variations is required for this type of sensor, causing inconvenience and inaccessibility in harsh sensing environments such as blood samples, which has dramatically limited the widespread clinical use of biosensors. In response, here, we have adopted E-AB sensors to achieve calibration-free measurements of small biological/drug molecules. Specifically, we employ one probe-attached redox reporter and a second intercalated redox reporter to generate two signals, achieving good sensor-to-sensor reproducibility and thus obviating the need for calibration. We first demonstrated the capability of E-AB sensors for the accurate measurement of kanamycin, tobramycin, and adenosine triphosphate (ATP) in phosphate-buffered saline (PBS) buffer, achieving concentration ranges of approximately 4.7 × 103-, 2.0 × 103-, and 12.7-fold, respectively. BSJ-4-116 cell line Then, we applied this calibration-free approach to the measurement of these three target molecules directly in undiluted serum, achieving a concentration precision of a few micromolars.Enhancing the reliability and sensitivity of gas sensors based on FETs has been of extensive concern for their practical application. However, few reports are available on nanofiber FET gas sensors fabricated by the electrospinning process. In this work, ethanol gas sensors based on Yb-doped In2O3 (InYbO) nanofiber FETs are fabricated by a simple and fast electrospinning method. The optimized In2O3 nanofiber FETs with a doping concentration of 4 mol % show a better electrical performance, including a high mobility of 6.67 cm2/Vs, an acceptable threshold voltage of 3.27 V, and a suitable on/off current ratio of 107, especially the enhanced bias-stress stability. When employed in ethanol gas sensors, the gas sensors exhibit enhanced stability and improved sensitivity with a high response of 40-10 ppm, which is remarkably higher than that of previously reported ethanol gas sensors. Moreover, the InYbO nanofiber FET sensors also demonstrate a low limit of detection of 1 ppm and improved sensing performance ranging from sensitivity to the ability of selectivity.