Drug-induced nephrotoxicity represents an important cause of acute kidney injury with associated patient morbidity and mortality and is often responsible for termination of drug development, after extensive resource allocation. We have developed a human kidney tubuloid system that phenocopies, in 3D culture, kidney proximal tubules, a primary injury site of most nephrotoxicants. Traditional end point assays are often performed on 2D cultures of cells that have lost their differentiated phenotype. Herein, we pair a tubuloid system with Nanoflare (NF) mRNA nanosensors to achieve a facile, real-time assessment of drug nephrotoxicity. Using kidney injury molecule-1 (KIM-1) mRNA as a model injury biomarker, we verify NF specificity in engineered and adenovirus-transfected cells and confirm their efficacy to report tubular cell injury by aristolochic acid and cisplatin. The system also facilitates nephrotoxicity screening as demonstrated with 10 representative anticancer moieties. 5-Fluorouracil and paclitaxel induce acute tubular injury, as reflected by an NF signal increase.In peatlands, bacterial tyrosinases (TYRs) are proposed to act as key regulators of carbon storage by removing phenolic compounds, which inhibit the degradation of organic carbon. Historically, TYR activity has been blocked by anoxia resulting from persistent waterlogging; however, recent events of prolonged summer drought have boosted TYR activity and, consequently, the release of carbon stored in the form of organic compounds from peatlands. Since 30% of the global soil carbon stock is stored in peatlands, a profound understanding of the production and activity of TYRs is essential to assess the impact of carbon dioxide emitted from peatlands on climate change. see more TYR partial sequences identified by degenerated primers suggest a versatile TYR enzyme community naturally present in peatlands, which is produced by a phylogenetically diverse spectrum of bacteria, including Proteobacteria and Actinobacteria. One full-length sequence of an extracellular TYR (SzTYR) identified from a soda-rich inland salt marsh has been heterologously expressed and purified. SzTYR exhibits a molecular mass of 30 891.8 Da and shows a pH optimum of 9.0. Spectroscopic studies and kinetic investigations characterized SzTYR as a tyrosinase and proved its activity toward monophenols (coumaric acid), diphenols (caffeic acid, protocatechuic acid), and triphenols (gallic acid) naturally present in peatlands.Two-dimensional (2D) MXene materials have attracted great attention as advanced energy storage devices. A Ti3C2 MXene film can be used as a high-performance electrode material for flexible supercapacitors owing to its high specific capacitance, excellent conductivity, and remarkable flexibility. Unfortunately, self-stacking of MXene nanosheets makes them hard to balance the volumetric and areal capacitance performance. Herein, high conductive polyaniline nanoparticles (PANI NPs, ∼10 nm) are proposed as intercalators to regulate the MXene nanosheet interlayer by the self-assembly method. Interlayered PANI NPs not only restrain MXene self-stacking but also enable more ion transport routes, and conductive PANI NPs filled in MXene interlayer are in the form of nanoparticles that can build interconnected conductive channels. Meanwhile, PANI NPs slightly changes the thickness of the MX/PANI NPs hybrid film, thus bringing a high volumetric capacitance. As a result, the freestanding MX/PANI NPs-10% electrode displays an excellent areal capacitance of 1885 mF cm-2 (377 F g-1), meanwhile maintains a high volumetric capacitance of 873 F cm-3 even when the load of MXene reaches 5 mg cm-2. Moreover, the symmetric supercapacitor assembled by MX/PANI NPs hybrid film demonstrates high areal energy density (90.3 μWh cm-2) and volumetric energy density (20.9 Wh L-1) compared to MXene-based symmetric supercapacitors reported in the literature. This rational design balancing areal and volumetric energy densities provides another approach for solving the inherent problems of MXene and further exploiting MXene materials toward application in advanced energy storage devices.exo-Methylene-β-lactams were synthesized in two steps from commercially available 3-bromo-2-(bromomethyl)propionic acid and reacted with arene diazonium salts in a Heck-type arylation in the presence of catalytic amounts of Pd(OAc)2 under ligand-free conditions. The products, arylidene-β-lactams, were obtained in high yields as single isomers. The β-hydride elimination step of the Pd-catalyzed coupling reaction proceeds with high exo-regioselectivity and E-stereoselectivity. With aryl iodides, triflates, or bromides, the coupling products were isolated only in low yields, due to extensive decomposition of the starting material at elevated temperatures. This underlines that arene diazonium salts can be superior arylating reagents in Heck-type reactions and yield coupling products in synthetically useful yields and selectivities when conventional conditions fail.A numerical approach is developed to capture the effect of rotation-vibration coupling in a practically affordable way. In this approach only a limited number of adjacent rotational components are considered to be coupled, while the couplings to other rotational components are neglected. This partially coupled (PC) approach permits to reduce the size of Hamiltonian matrix significantly, which enables the calculations of ro-vibrational states above dissociation threshold (scattering resonances) for large values of total angular momentum. This method is employed here to reveal the role of the Coriolis effect in the ozone formation reaction at room temperature, dominated by large values of total angular momentum states, on the order of J = 24 and 28. We found that, overall, the effect of ro-vibrational coupling is not minor for large J. Compared to the results of symmetric top rotor approximation, where the ro-vibrational coupling is neglected, we found that the widths of scattering resonances, responsible for the lifetimes of metastable ozone states, remain nearly the same (on average), but the number of these states increases by as much as 20%. We also found that these changes are nearly the same in symmetric and asymmetric ozone isotopomers 16O18O16O and 16O16O18O. Therefore, based on the results of these calculations, the Coriolis coupling does not seem to favor the formation of asymmetric ozone molecules and thus cannot be responsible for symmetry-driven mass-independent fractionation of oxygen isotopes.