UCBT induced a significant increase in the peak stress of cages and instruments, followed by CBT and UCBT-TFS, and the lowest mean values were observed for TT. Among the four different fixation techniques, TT offered the highest fixation strength and lowest implant stress, followed by UCBT-TFS and CBT, while UCBT was the least stable and resulted in increased stress of the screws and cages. UCBT-TFS improved biomechanical stability and appeared to be a less invasive alternative in well-selected patients with single-level TLIF.The purpose of this study was to construct and validate a model for predicting nonalcoholic fatty liver disease (NAFLD) in the non-obese Chinese population. A total of 13240 NAFLD-free individuals at baseline from a 4-y longitudinal study were allocated to a training cohort (n=8872) and a validation cohort (n=4368). The overall incidence of NAFLD was 13%. Nine significant predictors including age, gender, body mass index, fasting blood glucose, total cholesterol, triglycerides, high-density lipoprotein cholesterol, uric acid and alanine aminotransferase were identified and constructed for the nomogram using cox proportional hazards regression analyses. The concordance index was 0.804 and 0.802 in the training and validation cohorts, respectively. In the training cohort, the area under the ROC curve (AUC) for 1-y, 2-y, 3-y and 4-y risk was 0.835, 0.825, 0.816 and 0.782, respectively. Likewise, in the validation cohort, the AUC for 1-y, 2-y, 3-y and 4-y risk was 0.817, 0.820, 0.814 and 0.813. The calibration curves for NAFLD risk showed excellent accuracy in the predictive modeling of the nomogram, internally and externally. The nomogram categorized individuals into high- and low-risk groups, and the DCA displayed the clinical usefulness of the nomogram for predicting NAFLD incidence. Our nomogram can predict a personalized risk of NAFLD in the non-obese Chinese population. This nomogram can serve as a simple and affordable tool for stratifying individuals at a high risk of NAFLD, and thus serve to expedite treatment of NAFLD. This study aimed to find out the regulatory mechanism of miR-194-5p targeting STAT1/mTOR signaling pathway on the biological characteristics of endometrial epithelium cells from mice with endometriosis (EMs). Mouse model of EMs was constructed to observe the histopathological changes of endometrium HE staining. The targeting relationship between miR-194-5p and STAT1 was verified by bioinformatics website as well as dual-luciferase reporter assay. The expressions of miR-194-5p and STAT1 in the cells were detected by qRT-PCR, then, the proliferative activity, invasion ability, apoptosis and cycle of cells were determined after overexpression of miR-194-5p, or down-regulation of miR-194-5p and STAT1. In the ectopic endometrial epithelial cells, the expression of miR-194-5p was reduced, while the expression of STAT1 was significantly elevated. Overexpression of miR-194-5p or down-regulation of STAT1 significantly inhibited the proliferation and invasion and promoted apoptosis of ectopic endometrial cells. While down-regulation of miR-194-5p reversed the results, and inhibition of STAT1 partially reversed the effects partially. The miR-194-5p inhibits mTOR signaling pathway by inhibiting the expression of STAT1 gene, so as to inhibit the proliferation and invasion, as well as to promote the apoptosis of ectopic endometrial epithelial cells in mice with EMs.The miR-194-5p inhibits mTOR signaling pathway by inhibiting the expression of STAT1 gene, so as to inhibit the proliferation and invasion, as well as to promote the apoptosis of ectopic endometrial epithelial cells in mice with EMs.The incidence of ventricular arrhythmias (VAs) in chronic heart failure (CHF) exhibits a notable circadian rhythm, for which the underlying mechanism has not yet been well defined. Thus, we aimed to investigate the role of cardiac core circadian genes on circadian VAs in CHF. First, a guinea pig CHF model was created by transaortic constriction. Circadian oscillation of core clock genes was evaluated by RT-PCR and was found to be unaltered in CHF (P > 0.05). Using programmed electrical stimulation in Langendorff-perfused failing hearts, we discovered that the CHF group exhibited increased VAs with greater incidence at CT3 compared to CT15 upon isoproterenol (ISO) stimulation. Circadian VAs was blunted by a β1-AR-selective blocker rather than a β2-AR-selective blocker. see more Circadian oscillation of β1-AR was retained in CHF (P > 0.05) and a 4-h phase delay between β1-AR and CLOCK-BMAL1 was recorded. Therefore, when CLOCK-BMAL1 was overexpressed using adenovirus infection, an induced overexpression of β1-AR also ensued, which resulted in prolonged action potential duration (APD) and enhanced arrhythmic response to ISO stimulation in cardiomyocytes (P less then 0.05). Finally, chromatin immunoprecipitation and luciferase assays confirmed that CLOCK-BMAL1 binds to the enhancer of β1-AR gene and upregulates β1-AR expression. Therefore, in this study, we discovered that CLOCK-BMAL1 regulates the expression of β1-AR on a transcriptional level and subsequently modulates circadian VAs in CHF.Cartilage defects repair is still a challenge in clinical practice until now. Although many breakthroughs have been achieved in cartilage repair using tissue engineering technology, there are still no scaffolds available for large-scale clinical applications. Currently, fish collagen (FC) is a natural source that is considered as an alternative to mammal-derived collagen in engineering cartilage tissue due to its excellent biocompatibility, suitable biodegradability, lack of immunogenicity, rich sources, low cost and minimal risk of transmitting zoonoses, which implies great potential for use in cartilage regeneration. Herein, we successfully prepared three-dimensional porous FC scaffolds from three different concentrations of FC (0.5%, 1% and 2%) by freeze-drying technology. Our results indicated that increasing the FC concentration resulted in comparable levels of suitable biodegradability and good biocompatibility but lead to a concurrent decrease in pore size and porosity and a significant increase in water absorption capacity and mechanical properties; further, initial scaffold dimension was only sustained in the 2% FC concentration.