Journalists and experts contribute to the creation of frames (frame-building) of innovations. However, little is known about the specific contribution of these different societal actors to the frame-building of emerging information technologies. This article focuses on a specific emerging information technology - cyberinfrastructure for big data. In particular, we investigated the role of metaphors in the frame-building of cyberinfrastructure during its early development, and contrast the metaphorical framing of cyberinfrastructure by journalists in a corpus of US news texts (Study 1) with the metaphorical framing of experts in a corpus of interviews (Study 2). Results show considerable differences between the frames by journalists and experts in the frame-building process. Journalists, to a great extent, employ their own frames in conceptualizing cyberinfrastructure rather than drawing on the frames used by experts. Future research should investigate the impact of these different metaphorical frames on audience members. leaves are popularly used to treat asthma and diabetes. Despite the widespread consumption of this plant, there are few scientific studies regarding its toxicological potential. In order to conduct a thorough study concerning the potential adverse effects, the aim of this study was to assess acute and subacute toxicity tests of crude aqueous extract from leaves (CAE-Cp) using model, as well as cytotoxicity, genotoxicity and antioxidant activity. In addition, genotoxicity, and cytotoxicity of chlorogenic acid (CGA) and cytotoxicity of isoorientin (ISOO) were also evaluated. The antioxidant activity was verified by DPPH, cytotoxicity using sulforhodamine B (SRB) assay and genotoxicity by comet assay on V79 cells. The phytochemical analysis of CAE-Cp detected flavonoids and tannins, CGA and ISOO as the major compounds utilizing HPLC. The total flavonoid content (6.52mg/g EQ) and antioxidant activity (EC =62.15µg/ml) of CAE-Cp were determined. evaluations with CAE-Cp showed genotoxic effects at 0.31significant toxicity was observed in animals after 28 days. In view of the in vitro cytotoxicity and genotoxicity detected, further studies are necessary to establish the safe use of CAE-Cp.For the audiological assessment of the speech-in-noise abilities of children with normal or impaired hearing, appropriate test materials are required. Oligomycin A nmr However, in Denmark, no standardized materials exist. The purpose of this study was to develop a Danish sentence corpus suitable for testing school-age children. Based on the 600 validated test sentences from the Danish DAT (Dagmar, Asta, or Tine) corpus, 11 test lists comprising 20 sentences each were carefully constructed. These lists were evaluated in terms of their perceptual similarity and reliability with a group of 20 typically developing, normal-hearing children aged 6 to 12 years. Using stationary speech-shaped noise and diotic stimulus presentation, speech recognition thresholds (SRTs) were measured twice per list and participant at two separate visits. The analyses showed that six test lists were perceptually equivalent. These lists are characterized by a grand average SRT of -2.6 dB signal-to-noise ratio, a test-retest improvement of 0.6 dB, and a within-subject standard deviation of 1.1 dB signal-to-noise ratio. The other lists were characterized by slightly higher SRTs, slightly larger training effects, and slightly larger measurement uncertainty, but were otherwise also usable. Overall, it is therefore concluded that the developed corpus is suited for assessing speech recognition in noise in Danish 6- to 12-year olds. The corpus is publicly available.The reviews of this paper are available via the supplemental material section.Background. To determine face and construct validity for the new Bimanual Fundamentals curriculum for the Simendo® Virtual Reality Laparoscopy Simulator and prove its efficiency as a training and objective assessment tool for surgical resident's advanced psychomotor skills. Methods. 49 participants were recruited 17 medical students (novices), 15 residents (intermediates), and 17 medical specialists (experts) in the field of gynecology, general surgery, and urology in 3 tertiary medical centers in the Netherlands. All participants performed the 5 exercises of the curriculum for 3 consecutive times on the simulator. Intermediates and experts filled in a questionnaire afterward, regarding the reality of the simulator and training goals of each exercise. Statistical analysis of performance was performed between novices, intermediates, and experts. Parameters such as task time, collisions/displacements, and path length right and left were compared between groups. Additionally, a total performance score was calculated for each participant. Results. Face validity scores regarding realism and training goals were overall positive (median scores of 4 on a 5-point Likert scale). Participants felt that the curriculum was a useful addition to the previous curricula and the used simulator would fit in their residency programs. Construct validity results showed significant differences on the great majority of measured parameters between groups. The simulator is able to differentiate between performers with different levels of laparoscopic experience. Conclusions. Face and construct validity for the new Bimanual Fundamental curriculum for the Simendo virtual reality simulator could be established. The curriculum is suitable to use in resident's training programs to improve and maintain advanced psychomotor skills.This study is about proposing a vaccine for all four strains of dengue virus (DENV) that could be an important approach for reaching the WHO goal of reducing dengue morbidity and mortality. The significance of the DENV envelope proteins III lies in the fact that it elicits an immune response and hence can be a potential vaccine design candidate. This domain appears to play a key role in the host cell receptor binding for viral entry and in inducing long lasting protective immunity against the infection. We used long molecular dynamic simulation and mutagenesis scanning methods to provide the dynamic environment and propose the potential mutation that may result in enhancing the binding specificity and affinity of the antigen-antibody (Ag-Ab) complex. The binding free energetics were also estimated using free energy perturbation method. One charged mutation that is theorinine 93L to arginine interacting with epitopic glutamic acid 368 strongly contributing in increasing the binding affinity as well as specificity, predicted as -9.