Symptom-alleviating therapies for osteoarthritis (OA) management are inadequate. Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs, is limited due to associated side effects. We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA. Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions. It has shown promise in reducing pain and inflammation in preclinical models of arthritis. In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions. CBD was tested at 5, 10, 20, and 40 mg/kg orally and at 5% topically. Administered alone, CBD produced dose-correlated, statistically significant pain inhibition in all the models. Enhanced performance in pain and inflammation reduction was observed when CBD was orally administered in complex with the bioflavonoid compositions. Data from this study show that for clinically meaningful efficacy against OA, CBD may have to be delivered in higher dosage or formulated with other medicinal plants with similar activities.Maternal high-sweetener diet, such as sucrose, has been associated with an increased risk of metabolic and cognitive-related diseases in the offspring. This study was performed to determine the effect of maternal sweetener intake during gestation and lactation on learning and memory in adult female offspring rats. Twenty-eight female pups from dams fed standard diet (Control-C, n = 10), high-sucrose diet (HS-C, n = 6), and high-honey diet (Ho-C, n = 12) were fed standard diet after weaning and body weight and food intake were recorded once a week for 19 weeks. Learning and memory tests were conducted at week 14 (Y-maze) and 18 (Barnes maze). We found that birth weight of Control-C group was greater than the Ho-C (P  less then  .001). Blood glucose levels of the HS-C group were significantly higher than the Control-C and Ho-C groups. Control-C pups recognized the novel arm of the Y-maze compared with HS-C and Ho-C groups (P  less then  .01). Also, offspring of the HS-C group showed deficient performance in the Barnes test when compared with the Control-C and Ho-C groups (P  less then  .05). These results suggest that dams fed a high-sucrose diet during gestation and lactation favor high-glucose levels and deficient long-term memory performance in adult female offspring rats.Background Neck pain (NP), back pain (BP), and low back pain (LBP) are generally defined as "pain in the spine." With the increasing prevalence of childhood obesity, secondary problems such as pain in the spine have arisen. The purpose of this review was to investigate the relationship between body mass index (BMI) and pain in the spine in children or adolescents. Methods Publications were searched in PubMed, Web of Science, Scopus, and Google Scholar databases up to December 12, 2020. The search strategy in the database consisted of free text words and MeSH terms. Results Twelve studies were reviewed. It was determined that different methods were used in all 12 studies to evaluate pain. In the evaluation of overweight/obesity, these studies performed BMI assessment by dividing body weight in kilograms by height squared. Five studies showed a relationship between LBP and BMI, two studies showed a relationship between BP and BMI, and two studies showed a relationship between NP and BMI. Conclusions The review shows that there is a relationship between BMI and pain in the spine, especially LBP. There may be factors affecting this condition such as mechanical loading and hormonal metabolic activity in childhood and adolescence. Different methods are used in the studies in literature for the assessment pain in the spine and BMI, overweight, and obesity.Increased visceral adipose tissue (VAT) is associated with metabolic dysfunction, while subcutaneous adipose tissue (SAT) is considered protective. The mechanisms underlying these differences are not fully elucidated. This study aimed to investigate molecular differences in VAT and SAT of male Wistar rats fed a cafeteria diet (CD) or a standard rodent diet (STD) for three months. The expression of fatty acid metabolism genes was analysed by quantitative real-time PCR. Global and gene-specific DNA methylation was quantified using the Imprint® Methylated DNA Quantification Kit and pyrosequencing, respectively. Bodyweight, retroperitoneal fat mass, insulin resistance, leptin and triglyceride concentrations and adipocyte hypertrophy were higher in CD- compared to STD-fed rats. The expression of solute carrier family 27 member 3 (Slc27a3), a fatty acid transporter, was 9.6-fold higher in VAT and 6.3-fold lower in SAT of CD- versus STD-fed rats. Taqman probes confirmed increased Slc27a3 expression, while pyrosequencing showed Slc27a3 hypomethylation in VAT of CD- compared to STD-fed rats. The CD decreased global methylation in both VAT and SAT, although no depot differences were observed. Dysregulated fatty acid influx in VAT, in response to a CD, provides insight into the mechanisms underlying depot-differences in adipose tissue expansion during obesity and metabolic disease. click here Abbreviations CD cafeteria diet; E2F1 E2F Transcription Factor 1; EMSA electrophoretic mobility shift assay; EGFR epidermal growth factor receptor; GCF GC-Rich Sequence DNA-Binding Factor; HOMA-IR Homeostasis model for insulin resistance; NKX2-1 NK2 homeobox 1; PCR Polymerase chain reaction; qRT-PCR quantitative real-time PCR; RF retroperitoneal fat; SAT subcutaneous adipose tissue; Slc27a3 solute carrier family 27 member 3; STD standard diet; TNFα tumour necrosis factor alpha; TTS transcriptional start site; T2D Type 2 Diabetes; VAT visceral adipose tissue; WT1 I Wilms' tumour protein 1.