The findings of this work allow set targets in the process control of products using MH. Additionally, MCR-ALS detected an event not evidenced by conventional thermal analysis, the transformation of Form V to Form I.Toxic impurities were found in leucomycin and its preparation, however the content determination of impurities was challengeable due to the lacking of their reference standards. In this study, we developed high-performance liquid chromatography method coupled with charged aerosol detection (CAD) for the quantification of related substance of leucomycin (kitasamycin) bulk drugs and tablets, however, the CAD was not yet popular. In order to carry out quantitation work conveniently in the laboratory without CAD instruments, a high-performance liquid chromatography method coupled with ultraviolet (UV) detection was developed with the assistant of the HPLC-CAD results. The relative response of impurities on CAD chromatogram was used for guiding the establishment of HPLC-UV method, which could achieve the quantitation task in the absence of impurity reference standards. The developed HPLC-UV method was validated according to the ICH guideline and showed good precision, reproducibility and linearity with determination coefficient higher than 0.9999. The limit of detection and quantitation were 0.3 and 0.5 μg mL-1, respectively. The recoveries were 92.9 %-101.5 % at the spiked concentration levels of 0.1 %, 0.8 %, 1.0 and 1.2 % with relative standard deviations (RSDs, n = 3) lower than 2.0 %. Finally, the developed HPLC-CAD and -UV methods were compared by the determination of impurities in several batches of leucomycin bulk drugs and tablets. The results demonstrated that the developed HPLC-UV method was simple and reliable. This study developed methods to quantify the related substance in leucomycin and tablets, and discussed a strategy of the conversion of HPLC-CAD method to HPLC-UV method. The developed methods could be considered for implementation into pharmacopeial monographs in the future.LTP treatment was applied to induce a high-content alpha-linolenic acid (ALA) strain (C-12) from Chlorella sp. L166, the ALA content of C-12 was increased by 48.37%. The mechanism of LTP induction were examined. The results showed that LTP could facilitate pH change, induce malondialdehyde (MDA) production, cause protein leakage, and destroyed the microalgae cells. The genes of C-12 encoding pyruvate dehydrogenase (E2) were up-regulated 2.47-fold, and acetyl-CoA carboxylase (ACCase) down-regulated 0.48-fold compared to the wild type, these changed in the direction of ALA accumulation. Furthermore, the enzymes in DNA replication were significantly up regulated. Take ALA and biomass accumulation into account, LTP technology had a positive effect on ALA accumulation. Global view of metabolic variation also suggested that C-12 had an excellent adaptability to the changes of pH and peroxidation of LTP production. C-12 could be a promising ALA source of alternative materials for it do not compete with land.In this study, strategies for recovery of ammonia-stressed AD reactors were attempted, by addition of preserved bioaugmentation consortium in gel (BioG), fresh consortium in liquid medium (BioL), woodchip biochar (BW), and straw biochar (BS). In comparison to control group with ammonia, effective treatments, i.e., BioG, BioL, BW and BS raised the maximum methane production rate by 77%, 23%, 35%, and 24%, respectively. BW possibly acted as interspecies electrical conduits for Direct Electron Transfer based on conductivity and SEM analysis. BioG facilitated slow release of bioaugmentation inocula from gel into the AD system, which protected them from a direct environmental shock. According to microbial analysis, both BioG, BioL and BW resulted in increased relative abundance of Methanothermobacter thermautotrophicus; and BS induced selective raise of Methanosarcina thermophila. The increase of methanogens via these strategies led to the faster recovery of the AD process.Nutrient pollution released from highly accumulated swine wastewater is getting concerned due to global warming and waterbody harmful. Traditional combination of nitrification and denitrification is commonly applied to remove carbon and nitrogen compounds resided in various wastewater with disadvantages of high cost and energy requirements. Selleckchem SAR131675 This study applied the thermophilic flat panel photobioreactor (tFPBR) with high growth rate of TCL-1 culture to evaluate the efficiency of inorganic carbon and nitrogen transformation. This 12-h operation resulted that TCL-1 enriched batch, grown in 50 °C and alkaline environment with 1,000 µE/m2/s light intensity, had high potential for CO2 fixation rate of 122.29 ± 9.93 mg/L/h and nitrogen removal rate of 7.76 mg-N/L/h treating swine wastewater, in comparison with comprehensive community involved in carbon and nitrogen cycles in the field-scale anoxic tank. This study provided the Rapid-growing photosynthetic cyanobacteria in place of slow-growing autotrophic microbes for of carbon and nitrogen transformation in the wastewater system.A kind of gel beads loaded with iron-carbon powder (Fe-C), Zoogloea sp. L2, and riboflavin (VB2) were prepared through cross-linking of sodium alginate (SA) to establish an immobilized bioreactor. The optimal ratio of SA beads was adjusted by orthogonal experiment. The change of oxidation-reduction potential (ORP) and the concentration of Fe2+ and Fe3+ showed that the addition of VB2 as a redox mediator can promote denitrification. Under the optimal conditions (carbon to nitrogen (C/N) ratio = 2.0, pH = 7.0, and hydraulic retention time (HRT) = 8 h), the nitrate removal efficiency (NRE) of bioreactor reached 98.48% (1.99 mg L-1h-1). Furthermore, Fourier transform infrared spectrometer (FTIR), Fluorescence excitation-emission matrix (EEM), X-ray diffraction (XRD), and gas chromatography (GC) analysis revealed that the immobilization and denitrification of the immobilized bioreactor were excellent. High throughput sequencing also showed that Zoogloea played a vital role in nitrate removal. Qingchang Suppository, a formula used for more than 30 years in Longhua Hospital, has shown satisfactory clinical effects on Ulcerative Colitis (UC). However, its therapeutic mechanism has not been fully elucidated. The study aims to investigate the effects of Qingchang Suppository powder (QCSP) and its ingredients by regulating the IL-17A signaling pathway which plays an important role in the development of UC. HPLC was used to analyze the main ingredients (Gallic acid, Indigo, Indirubin) in QCSP. HT-29 cells were induced by rhIL-17A and TNF-α, and IL-17A related protein expressions were determined by western blot. BALB/C mice were induced by 4% Dextran Sodium sulfate (DSS). The effects of QCSP and its ingredients were evaluated by measuring weight loss, disease activity index (DAI), colon length, histological analysis. Western blot was used for analysis of IL-17A and MAPK related proteins p-ERK, p-JNK, p-P38. Quantitative reverse transcription polymerase chain reaction (q-PCR) was used to detect the expression of IL-17A, HSP90 and ACT1 in colon tissue.